| 反相高效液相色谱法检测胎盘中的新洁尔灭 |
| |
| 引用本文： | 周艳彬,马岿,李叶红,朱宏增,马运荣,赵新静. 反相高效液相色谱法检测胎盘中的新洁尔灭[J]. 中外医疗, 2013, 32(3): 15-16 |
| |
| 作者姓名： | 周艳彬 马岿 李叶红 朱宏增 马运荣 赵新静 |
| |
| 作者单位： | 济宁市妇幼保健院;济宁市第一人民医院;济宁市药检所 |
| |
| 基金项目： | 济宁市科技局基金项目:济科字[2010]85-48号 |
| |
| 摘 要： | 目的应用反相高效液相色谱法（RP-HPLC）测定胎盘中的新洁尔灭。采用甲醇浸泡溶解,10%高氯酸沉淀蛋白的方法,提取样品后进行高效液相色谱测定。方法色谱柱：用Acclaim C18色谱柱（250mm×4.6mm i.d.,5μm）;流动相：甲醇-乙腈-水（53：20：27）;紫外检测器：检测波长：254nm;柱温：30℃;流速：0.85mL/min。结果在2.0～10.0μg·mL-1质量浓度范围内,呈现良好线性关系（r：0.9999）,回收率为95.7%～99.6%,日内RSD为1.4%～1.8%,日间RSD为1.5%～2.1%。结论该方法简便、快速、灵敏、准确,完全符合中国药典2010版规定,可作为临床检测方法。
|
| 关 键 词： | 高效液相色谱法 新洁尔灭 胎盘 |
Using Reverse Phase High Efficiency Liquid Chromatography to Examine the Bromogeramine in Placenta |
| |
| ZHOU Yangbin,MA Kui,LI Yehong,ZHU Hongzeng,MA Yunrong,ZHAO Xinjing. Using Reverse Phase High Efficiency Liquid Chromatography to Examine the Bromogeramine in Placenta[J]. China Foreign Medical Treatment, 2013, 32(3): 15-16 |
| |
| Authors: | ZHOU Yangbin MA Kui LI Yehong ZHU Hongzeng MA Yunrong ZHAO Xinjing |
| |
| Affiliation: | 1.Mental and Child Health Hospital of Jining, Shangdong province, Jining 272000, China; 2.Jining First People’s Hospital, Shangdong province, Jining 272000, China; 3.Jining Institute for Drug Control, 272000, China; |
| |
| Abstract: | Objective Apply the HPLC to determine Bromogeramine in the placenta. Use methanol to dissolve andl0% Perchloric acid to subside protein to get the sample which is tested by HPLC. Methods Column： Acclaim C18 （250mm×4.6mm i.d.,5μm） mobile phase methanol： Methanol： Acetonitrile： water （53：20：27） wave length：254nm column temperature 30℃ flow rate： 0.85 ml/ min. Results The during concentration range 2.0-10.0 μg·mL-1 shows a good linear relationship （r：0.9998）. recovery rate is 95.7% -99.6%, Intra-day RSD isl.4%-1.8%, Inter-day RSD is 1.5% - 2.1%. Conclusion The method is simple, rapid, sensitive, accu- rate and fully complies with the provisions of the Chinese Pharmacopoeia 2005 edition, which can be used as clinical testing method. |
| |
| Keywords: | HPLC Ethacridine lactate amniotic fluid Placenta |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
