| 一株柯萨奇病毒B组5 型(Cox.B5) 病毒的分离及VP1基因分析 |
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| 引用本文： | 李华,杨卉娟,柯华昕,陈俊英,潘玥,赵玉娇,孙强明,马绍辉. 一株柯萨奇病毒B组5 型(Cox.B5) 病毒的分离及VP1基因分析[J]. 医学研究杂志, 2011, 40(9): 55-58 |
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| 作者姓名： | 李华 杨卉娟 柯华昕 陈俊英 潘玥 赵玉娇 孙强明 马绍辉 |
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| 作者单位： | 中国医学科学院/北京协和医学院医学生物学研究所 云南省重大传染病疫苗研发重点实验室, 昆明,650118 |
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| 摘 要： | 目的研究2009年昆明市无菌性脑膜炎的病原柯萨奇B5(coxsackie virus B5,CVB5)分离株(KMA193-09)的VP1基因特征。方法采用RD细胞、Hep-2细胞对患者粪便标本进行病毒分离,反转录-聚合酶链反应(RT-PCR)扩增病毒VP1基因并进行序列测定,用Mega 4.0等软件分析处理。结果从无菌性脑膜炎患者粪便标本中,分离到CVB5,其VP1区的核苷酸长度均为831bp,未发现核苷酸插入与丢失。与浙江COXB5/ZHEJIANG/12/02(CFS)株、山东02336/SD/CHN/2002/CB5株及浙江COXB5/ZHEJIANG/13/02株氨基酸同源性最高为98.19%,与国外毒株的同源性为95.67%～97.83%。在进化树上与YZ081/SD/CHN/2005/CB5株显示在同一个分支上。结论分离的肠道病毒为柯萨奇病毒B组5型(CVB5),分离株(KMA193-09)VP1区变异较小。
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| 关 键 词： | 无菌性脑膜炎 柯萨奇病毒B组5型(Cox.B5) VP1基因 分析 |
| 收稿时间： | 2011-02-24 |
Analysis of VP1 Gene of Coxsackievirus B5 for Aseptic Meningitis Prevailing Isolated from Kunming in 2009 |
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| Li Hua,Yang Huijuan,Ke Huaxin,Chen Junying,Pan Yue,Zhao Yujiao,Shun Qiangming,Ma Shaohui. Analysis of VP1 Gene of Coxsackievirus B5 for Aseptic Meningitis Prevailing Isolated from Kunming in 2009[J]. Journal of Medical Research, 2011, 40(9): 55-58 |
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| Authors: | Li Hua Yang Huijuan Ke Huaxin Chen Junying Pan Yue Zhao Yujiao Shun Qiangming Ma Shaohui |
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| Affiliation: | Li Hua,Yang Huijuan,Ke Huaxin,et al.Institute of Medical Biology,Peking Union Medical College,Chinese Academy of Medical Science,Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Disease,Yunnan 650118,China |
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| Abstract: | Objective To analyze the genetic characterization of the VP1 gene of coxsackie virus B5 causing meningitis isolated from Kunming in 2009.Methods The specimens of stool were isolated in Hep-2 and RD cell lines.Viral RNA was extracted and the VP1 gene was amplified using RT-PCR and sequenced.The sequence of the VP1 gene was compared with VP1 gene sequences of other coxsackie virus from Genbank using Mega4.0 software.Results The VP1 gene of coxsackie virus B5 isolated from stool specimens was 831 nucleotides.T... |
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| Keywords: | Aseptic meningoencephalitis Coxsackie virus B5 VP1 gene Analyze |
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