三联放大技术对HBV核酸检测的研究

目的：建立聚合酶链反应偶联印迹杂交和酶联放大技术（即三联放大技术）对乙型肝炎病毒的检测方法，探索适用于血液筛查乙肝的核酸检测技术。方法：通过检测HBV定值标准血清，评估三联放大技术检测血清HBVDNA的灵敏度。然后，应用三联放大技术对乌鲁木齐市500人（次）血清学检测病毒标志物阴性的献血者进行HBVDNA检测。结果：建立三联放大技术HBV检测体系，该体系对90IU／ml的HBV标准血清检出率为100％，对45IU／ml的HBV标准血清检出率为75％。对500人（次）血清学检测病毒标志物阴性的献血者，未检出HBVDNA阳性。结论：三联放大技术对HBV检测灵敏度较高，可运用于血液筛查乙型肝炎病毒。

Study of triple amplification technology detect HBV

Objective:To detect hepatitis B virus in serum by establishing the polymerase-chain reaction coupled with blot-hybridization and enzyme-linked technology(triple amplification technology).Discuss that nucleic acid amplification testing applies to detect HBV in blood donors screening.Methods:Triple amplification technology was used to detect HBV standard serum to evaluate the sensitivity of triple amplification technology detected HBV DNA in serum.500 seronegative samples were detected by triple amplification...