|
|||||
|
|
| 两种校准品和3种双缩脲试剂测定血清总蛋白结果偏倚评估及方法学评价 | |
| 引用本文： | 王时南,杨欢. 两种校准品和3种双缩脲试剂测定血清总蛋白结果偏倚评估及方法学评价[J]. 医学研究杂志, 2011, 40(10): 125-128 |
| 作者姓名： | 王时南 杨欢 |
| 作者单位： | 325000,浙江省温州市第二人民医院/温州市肿瘤医院 |
| 摘 要： | 目的评估两种校准品及3种双缩脲试剂盒测定血清总蛋白结果的偏倚,评价3种双缩脲试剂盒血清总蛋白的精密度、线性和灵敏度。方法用3种双缩脲试剂盒及两种校准品组合成6个检测系统,按NCCLS EP9-A文件,评估3种双缩脲试剂盒及两种校准品测定结果偏倚和统计学分析;按NCCLS EP5-A文件,评价3种双缩脲试剂盒批内、批间、日间和总CV%。按NCCLS EP6-P文件评价3种双缩脲试剂盒的线性;比较3种双缩脲试剂盒测定同一份血清在540nm处的吸光度。结果用Roche校准血清(c.f.a.s)校准仪器,双缩脲试剂盒B、C与试剂盒A比较,差异无统计学意义(P>0.05);用总蛋白标准液校准仪器,试剂盒B与试剂盒A比较,差异无统计学意义(P>0.05),试剂盒C与试剂盒A比较,差异具有统计学意义(P<0.01);3种双缩脲试剂盒测定血清总蛋白总CV为0.86%～1.16%;波长为540nm,试剂盒A的吸光度明显高于试剂盒B、C;试剂盒A和B线性良好(F |
| 关 键 词： | 双缩脲试剂 血清总蛋白 精密度 线性 偏倚 生化分析仪 |
| 收稿时间： | 2010-12-15 |
| 修稿时间： | 2011-01-07 |
Bias Analysis and Methodological Evaluation on Test Results of Serum Total Protein with 2 Calibrators and 3 Biuret Reagents |
|
| Wang Shinan,Yang Huan. Bias Analysis and Methodological Evaluation on Test Results of Serum Total Protein with 2 Calibrators and 3 Biuret Reagents[J]. Journal of Medical Research, 2011, 40(10): 125-128 | |
| Authors: | Wang Shinan Yang Huan |
| Affiliation: | Wang Shinan,Yang Huan.Department of Clinical Laboratory,Wenzhou 2nd Hospital,Wenzhou Tumor Hospital,Zhejiang 325000,China |
| Abstract: | Objective To analyze test bias of serum total protein with 2 calibrators and 3 biuret reagents,and to evaluate precision,linearity and sensitivity of the 3 biuret reagents and 2 calibrators.Methods Three biuret reagents and 2 calibrators consist of 6 test systems.According to EP9-A(NCCLS),test bias of 3 biuret reagents and 2 calibrators was analyzed statistically;according to EP5-A,the results of intra-batch,between batch and batches on different days,and total CV% of 3 biuret reagents analyzed;according to... |
| Keywords: | Biuret reagent Serum total protein Precision Linearity Bias Biochemical analyzer |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《医学研究杂志》浏览原始摘要信息 | |
| 点击此处可从《医学研究杂志》下载全文 | |