醛固酮对h9c2心肌细胞缝隙连接蛋白43的影响

目的：观察醛固酮对h9c2心肌细胞缝隙连接蛋白43（Connexin 43,Cx43）表达的影响。方法：将h9c2心肌细胞随机分为对照组和实验组,实验组用不同浓度（1×10^-8、1×10^-6、1×10^-4 mol/L）醛固酮处理24 h后,用RT-PCR和Western Blot观察不同浓度醛固酮下h9c2心肌细胞Cx43蛋白和mRNA的变化。结果：1×10^-8 mol/L醛固酮组Cx43mRNA和蛋白表达含量较对照组增加;1×10^-6 mol/L和1×10^-4 mol/L组的Cx43蛋白表达含量较对照组减少,而Cx43mRNA较对照组无明显改变。结论：醛固酮可能是通过对表达Cx43的双重影响来参与心肌细胞间隙连接重构的。

Effects of aldosterone on Cx43 gap junction expression in h9c2 cardiomyocytes

Objective：To investigate the effects of aldosterone on the expression of Cx43 in h9c2 cells.Methods：The h9c2 cells were randomly divided into control group and experimental group.The experimental group was exposed to various concentration of aldosterone（1×10^-8,1×10^-6,1×10^-4mol/L） for 24 hours,the changes of Cx43 protein and Cx43 mRNA were investigated by Western Blot and RT-PCR.Results：Compared with the control group,the expression of Cx43 protein and Cx43 mRNA increased in 1×10^-8mol/L aldosterone group,but the Cx43 protein decreased in 1×10^-6 mol/L and 1×10^-4 mol/L aldosterone group,the Cx43 mRNA was no obvious difference.Conclusion：Aldosterone may be involved in gap junction remodeling through its dual effects on the expression of Cx43.