核心蛋白聚糖对人肺腺癌细胞生长及转移的抑制作用研究

 目的 探讨核心蛋白聚糖（Decorin）对人肺腺癌细胞株A549细胞生长及转移的影响。方法 利用MTT法检测不同浓度不同时间Decorin对A549细胞的杀伤活性，流式细胞仪检测分析Decorin对A549细胞周期及凋亡的影响，体外黏附实验、侵袭实验和迁移实验检测Decorin对A549细胞黏附、侵袭及迁移能力的影响。结果 Decorin在体外能够抑制A549肿瘤细胞增生，且这种增生抑制作用呈剂量和时间依赖性。Decorin在体外能够诱导A549肿瘤细胞凋亡，作用与浓度和时间相关。Decorin 30 μg／ml在体外作用于A549细胞可使A549细胞黏附率下降，迁移细胞数和侵袭细胞数均减少。结论 Decorin在体外能够抑制人肺腺癌A549肿瘤细胞生长，诱导其凋亡，并通过对肿瘤转移多个步骤的抑制而发挥抗转移作用。

Studies of suppression ability of Decorin on metastasis of adenocarcinoma cell

Objective In order to further elucidate the anti-metastatic mechanism of Decorin, A549 cells were cultured with decorin in vitro. Methods Adenocarcinoma cell A549 lines were cultured with decorin in a wide range concentration for various time. Cell activity were studied by MTT.The changes of cell cycle were analyzed by FCM, Adhesion assay, invasion assay, migration assay technique were used to detect the adhesin,invasion and migration ability of decorin on A549. Results The proliferation of A549 cell could be inhibited by decorin. The inhibition effect showed the time-dose relationship. Apoptosis of adenocarcinoma cell lines could be efficiently induced by decorin in a time/dose-dependent manner. 30 μg/ml decorin could decrease adhesive ratio and the ability of invasion or migration in A549 cells. Conclusion Decorin can in－hibit adenocarcinoma cell lines proliferation and induce apoptosis of adenocarcinoma cells. Decorin may play an anti-metastatic role in several steps.