分泌型卷曲相关蛋白2对HepG2细胞生物学行为的影响

目的 了解分泌型卷曲相关蛋白2(sFRP2)对HepG2细胞增殖、侵袭和迁移等生物学行为的影响.方法 采用sFRP2重组腺病毒感染HepG2细胞,四甲基偶氮唑盐法检测HepG2细胞增殖,流式细胞术检测细胞周期分布,免疫组织化学法检测肿瘤转移相关因子的表达,Westernblot检测β连环素的表达,Tmnswell小室检测细胞迁移能力.结果 sFRP2明显抑制HepG2细胞增殖,限制细胞周期从Go/G<,1>期进入S期;sFRP2显著增强nepG2细胞CD44和CD82/KAI1等与肿瘤转移抑制相关蛋白的表达,而明显降低有助于肿瘤侵袭转移的细胞外基质金属蛋白酶诱导因子的表达; sFRP2可降低HepG2细胞的迁移能力.sFRP2感染前后,HepG2细胞均有β连环素的表达,且其表达差异无统计学意义.结论 sFRP2的重组腺病毒能成功感染HepG2细胞,并对H印G2细胞的增殖、侵袭和转移具有抑制效应.

Effect of sFRP2 on the biological behavior of HepG2 cells

Objective To investigate the effect of sFRP2 on the biological behavior of human hepatoma carcinoma HepG2 cells. Methods HepG2 cells were infected with recombinant adenovirus con-raining mouse sFRP2 gene, and then the proliferation, cell cycle distribution, expression of tumor metastasis related factors (CD44, CD82/KAII, EMMPRIN) and beta-catenin protein, and migration abifity of the cells were detected by MTT, FCM, immunohistochemistry, Western blot and Transwell inserts, respectively. Re-sults sFRP2 protein inhibited the proliferation of HepG2 cells, and increased the percentage of G<,0>/G<,1> period cells. Expression of CD44 and CD82/KAI1 proteins, which could inhibit invasion and metastasis of tumor cells, was upregulated. However, EMMPRIN protein, which could promote the above properties of tumor cells decreased in HepG2 cells infected with the recombinant adenovirus containing mouse sFRP-2 gene. Western blot demonstrated that bcta-catenin was expressed in HepG2 cells and there was no significant differ-ence between the treated and the control groups. Transwell insert test showed sFRP2 protein decreased the migration ability of HepG2 cells. Conclusion The recombinant adenovirus containing mouse sFRP-2 gene could infect HepG2 cells, sFRP2 protein could significantly reduce the capability of proliferation, invasion and metastasis of HepG2 celts.