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| 应用酵母双向杂交系统筛选和鉴定P75NGFR作用的蛋白 | |
| 引用本文： | 简忠 黄秉仁. 应用酵母双向杂交系统筛选和鉴定P75NGFR作用的蛋白[J]. 中国冶金工业医学杂志, 2000, 17(3): 129-131 |
| 作者姓名： | 简忠 黄秉仁 |
| 作者单位： | [1]首钢总医院神经内科 [2]中国协和医科大学基础医学院分子生物学国家重点实验室 |
| 摘 要： | 目的：探讨Ｐ７５ＮＧＦＲ介导神经细胞凋亡的分子机制。方法；以转染了Ｐ７５ＮＧＦＲ的大鼠小脑神经细胞株Ｒ２Ｌ２为细胞模型，应用酵母双向杂交系统（ｔｗｏ－ｈｙｂｒｉｄ ｓｙｓｔｅｍ）筛选和鉴定Ｐ７５ＮＧＦＲ胞内区Ｐ７５ＩＣＤ作用的蛋白。结果：和Ｐ７５ＮＧＦＲ作用的蛋白由一个６００ｂｐ大小的ＣＤＮＡ片段编码。结论：该工作将有助于探讨Ｐ７５ＮＧＦＲ介导细胞凋亡的分子机制和信号，为勃勃系统退行性病变的病因、 |
| 关 键 词： | 酵母双向杂交系统 神经生长因子 p75NGFR |
Screening and identification of p~(75) NGFR ligands by two-hybrid system |
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| Jian Zhong,Huang Bingren,Xue Qiming. Screening and identification of p~(75) NGFR ligands by two-hybrid system[J]. Chinese Medical Journal of Metallurgical Industry, 2000, 17(3): 129-131 | |
| Authors: | Jian Zhong Huang Bingren Xue Qiming |
| Affiliation: | Jian Zhong,Huang Bingren,Xue Qiming.General Hospital of Shoudu Iron and Steel Company.Beijing 100041 |
| Abstract: | Objective:To explore the mechanism of p75 NGFR signals in neural cell apoptoic process.Methods:Two-hybrid system was used to screen a library for genes encoding a novel protein that interacted with the intracellular domain of p75 NGFR .The library was constructed by using cDNA from p75 NGFR -expressing R 2L 1 cells in a serum-free medium to induce apoptosis.Results:A 600 bp cDNA fragment encoded a novel protein that interacted with p75 ICD.Conclusions:The study is beneficial to exploring how the neurodegenerative disorders and brain aging occur,and how to control them. |
| Keywords: | Two-hybrid system p~(75) NGFR |
| 本文献已被 CNKI 维普 等数据库收录！ | |