基于组合相似度丹参样本质量稳定性评价

目的:基于组合相似度进行丹参样本质量的稳定性评价。方法:采集丹参水提液的色谱指纹图谱数据,建立组合相似度,基于样本间组合相似度、共有峰夹角余弦相似度和所有峰夹角余弦相似度对9个丹参样本进行系统聚类分析,对丹参样本质量的稳定性进行评价。结果:9个丹参样本的组合相似度与所有峰夹角余弦相似度的计算值接近,2种相似度方法的系统聚类结果与丹参色谱指纹图谱的主成分分析结果一致,结果表明,9个丹参样本较好地聚为2类。结论:组合相似度可用于9个丹参样本峰比例稳定性的初步评价,为原料药质量的稳定性评价提供一种相似度方法。

Quality stability evaluation of Radix Salviae Miltiorrhizae by applying combination similarity

Objective: To investigate the application of combination similarity to quality stability evaluation.Methods:The chromatographic fingerprints of Radix Salviae Miltiorrhizae were collected and combination similarity was established by the chromatographic peaks area of 9 samples of Radix Salviae Miltiorrhizae.The quality stability of 9 samples of Radix Salviae Miltiorrhizae were evaluated by the HCA(Hierarchical Cluster Analysis) based on combination similarity,cosine of common peaks and cosine of all peaks between every two samples.Results: The calculated results of combination similarity was close to cosine of the angle calculating all peaks area for all 9 samples of Radix Salviae Miltiorrhizae.The PCA(Principle Component Analysis) result of 9 samples confirmed the HCA result of 9 samples based on combination similarity and based on cosine of the angle calculating all peaks area,which 9 samples of Radix Salviae Miltiorrhizae were classified into 2 groups.Conclusion:Combination similarity could preliminary evaluate the peaks proportion stability of 9 samples of Radix Salviae Miltiorrhizae and becomes an alternative similarity measure to evaluate the quality stability of Chinese crude drug.