电针刺大鼠足三里穴对应激大鼠延髓内脏带内Fos和GFAP表达的影响

目的探讨延髓内脏带（MVZ）中即刻早期基因Fos和胶质原纤维酸性蛋白（GFAP）在针刺足三里穴保护应激大鼠胃黏膜中的表达及其意义。方法雄性SD大鼠24只，随机分为空白对照组、应激组、电针组，每组8只。利用免疫组织化学方法，观察Fos和GFAP在中枢延髓内脏带内孤束核（NTS）、迷走神经背运动核（DMV）中的表达并计算各组的溃疡指数（UI）。结果Fos阳性神经元和GFAP阳性星形胶质细胞表达主要位于延髓内脏带内NTS、DMV中。应激组与空白对照组比较，UI明显上升（P〈0．01），Fos和GFAP在中枢延髓内脏带内大量表达。电针组与应激组比较，Fos和GFAP在中枢延髓内脏带内的表达下调，UI下降（P〈0．01）。结论电针刺足三里穴对应激大鼠胃黏膜损伤具有保护作用，其机制可能与其调节延髓内脏带内免疫阳性神经元和星形胶质细胞的功能活动有关。

Effects of acupuncture at Tsusanli (He-Sea Point) on expression of FOS and GFAP in medullary visceral zone of rats under stress

Objective To study the expression of Fos gene and GFAP in medullary visceral zone (MVZ) of rats under stress with their gastric mucosa protected by acupuncture. Methods 24 male SD rats were randomly divided into 3 groups: control, stress and acupuncture group, with 8 for each group. Immunohistochemical methods were employed to detect the expression of Fos and GFAP in the nucleus of solitary tract (NTS) and dorsal motor nucleus of vagus (DMV) of medullary visceral zone. The extent of gastric mucosal damage was evaluated by ulcer index (UI). Results Fos-positive neurons and GFAP-positive stellar microgliocytes were found mainly in NTS and DMV of the medullary visceral zone. Compared with control group, the expression of Fos and GFAP was greatly up-regulated in the stress group, and UI increased obviously (P<0.01). Compared with the stress group, both the Fos and GFAP expression and the UI was down-regulated in acupuncture group (P<0.01). Conclusion Acupuncture at Tsusanli (He-Sea Point) can protect gastric mucosa of rats under stress. The regulative function to Fos and GFAP immunoreactive neurons and microglial cell in medullary visceral zone may be involved in the mechanism of the protective effect.+