高效液相色谱法测定不同产地夏枯草中咖啡酸和迷迭香酸的含量

目的比较不同产地夏枯草中咖啡酸和迷迭香酸的含量。方法采用高效液相色谱法，Waters SunFire C18色谱柱（4．6mm×150mm，5μm），甲醇-0．1％甲酸梯度洗脱，流速1．0mL／min，检测波长330nm。结果咖啡酸在0．0992～0．4960gg范围内与峰面积呈良好的线性关系（r=0．9999），平均回收率为99．75％，RSD=2．27％（n=5）；迷迭香酸在0．8288～4．1440gg范围内与峰面积呈良好的线性关系（r=0．9998），平均回收率为101．37％，RSD=1．19％（n=5）；不同产地夏枯草中咖啡酸与迷迭香酸的含量分别在0．02％-0．05％，0．09％-0．20％之间。结论不同产地夏枯草中咖啡酸和迷迭香酸含量差异很大，使用夏枯草时要注意产地差异。

Determination of Caffeic Acid and Rosmarinic Acid in Prunella vulgaris from Different Habitat by HPLC

Objective To establish an HPLC method for determination and comparison of the content of caffeic acid and rosmarinic acid in Prunella vulgaris from different habitat. Methods The determination was carried out with Waters Zorbax SunFire C18 （4.6 mm× 150 mm, 5μm）, gradient MeOH-0.1% acetic acid solution was taken as the mobile phase at a flow rate of 1.0 mL/min, and the detection wavelength was at 330 nm. Results The linear range of caffeic acid was 0.0992-0.4960 μg （r =0.9999）. The average recovery rate of caffeic acid was 99.75% （RSD=2.27%, n =5）. The content of caffeic acid in Prunella vulgaris from different habitat ranged from 0.02% to 0.05%. The linear range of rosmarinic acid was 0.828 8-4.144 0 μg （r=0.999 8）, The average recovery rate of rosmarim＇c acid was 101.37% （RSD= 1.19%, n =5）. The content of rosmarinic acid in Prunella vulgaris from different habitat ranged from 0.09% to 0.20%. Conclusion The content of caffeic acid and rosmarinic acid in Prunella vulgaris from different habitat varied greatly.