基环氧合酶-2和诱导型一氧化氮合酶在舌不典型增生和鳞癌组织中的表达

背景与目的:近期研究表明,环氧合酶(cyclooxygenase-2,COX-2)和诱导型一氧化氮合酶(induciblenitricoxidesynthase,iNOS)共同参与肿瘤的发生发展过程,但有关它们异常表达与舌鳞癌生物学行为的关系尚不清楚。本研究拟探讨COX-2和iNOS在舌鳞癌的表达和两者间的相互关系。方法:应用免疫组化SP法,检测59例舌鳞癌及其45例增生性病变(轻、中、重度不典型增生分别为22例、20例、3例)和36例癌旁正常鳞状上皮中COX-2蛋白、iNOS蛋白的表达情况,并对这些指标进行相关分析。结果:在癌旁正常鳞状上皮、轻、中、重度不典型增生上皮和舌鳞癌组织中,COX-2蛋白异常表达检出率分别为8.3%(3/36)、4.5%(1/22)、5.0%(1/20)、0(0/3)和45.8%(27/59);iNOS蛋白异常表达检出率分别为44.4%(16/36)、72.8%(16/22)、80.0%(16/20)、100.0%(3/3)和98.3%(58/59)。COX-2蛋白在癌旁正常鳞状上皮的表达,与舌鳞癌组织的表达差异有显著性(P<0.001),与不典型增生总体的表达差异无显著性(P>0.05);iNOS蛋白在癌旁正常鳞状上皮的表达,与不典型增生总体和癌组织的表达差异均有显著性(P<0.001)。等级相关分析结果显示,COX-2、iNOS表达异常与组织学分级均显著正相关(相关系数r分别为0.418和0.607,P值均<0.001),而且COX-2蛋白与iNOS蛋白之间也具有显著相关性(r=0.245,P<0.001)。结论:COX-2和iNOS蛋白异常表达与舌鳞癌癌变过程显著相关。

Expression of Cyclooxygenase-2 and Inducible Nitric Oxide Synthase in Tongue Hyperplasia and Tongue Squamous Cell Carcinoma

BACKGROUND & OBJECTIVE: Recently, it has been recognized that both cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) produce important endogenous factors of human tumor progression. However, the biological significance of the adnormal expression of COX-2 and iNOS in tongue squamous cell carcinoma remains unclear. This study was to investigate the expression of COX-2 and iNOS in tongue squamous cell carcinoma and precancerous lesions, and analyze their interrelation. METHODS: The expression of COX-2 and iNOS in 59 specimens of tongue squamous cell carcinoma (SCC), 45 specimens of hyperplasia (22 cases of mild hyperplasia, 20 cases of moderate hyperplasia, and 3 cases of severe hyperplasia), and 36 specimens of pericancerous normal epithelium was detected by SP immunohistochemistry. RESULTS: The positive rates of COX-2 were 8.3% in normal epithelium, 4.5% in mild hyperplasia, 5.0% in moderate hyperplasia, 0 in severe hyperplasia, and 45.8% in SCC, respectively; those of iNOS were 44.4% in normal epithelium, 72.7% in mild hyperplasia, 80.0% in moderate hyperplasia, 100% in severe hyperplasia, and 98.3% in SCC, respectively. The positive rates of COX-2 and iNOS were significantly higher in SCC than in normal epithelium (P<0.001); the positive rate of iNOS was also significantly higher in hyperplasia than in normal epithelium (P<0.001). The overexpression of COX-2 and iNOS was positively correlated with pathologic grade of the lesions (r=0.418, P<0.001; r=0.607, P<0.001). COX-2 expression was positively correlated with iNOS expression (r=0.245, P<0.001). CONCLUSOIN: The overexpression of COX-2 and iNOS is closely related to the carcinogenesis of the tongue.