白细胞介素-18基因单核苷酸多态性及其血清含量与溃疡性结肠炎的相关性

目的 探讨白细胞介素(IL)-18基因单核苷酸多态性(SNP)及其血清含量与溃疡性结肠炎(UC)的相关性.方法 采用PCR扩增和直接测序法检测50例UC患者及128名健康对照者的IL-18基因5'端4个SNP位点(rs187238 G/C、rs5744228 G/A、rs360718 A/C、rs360717 G/A),同时检测受试者血清IL-18含量.结果 UC组血清IL-18含量明显高于对照组,两组间差异有统计学意义(3029.9±111.7)pg/ml比(133.2±39.4)pg/ml,(P<0.01).rs187238 C、rs360718 C、rs360717 A位点与UC显著相关(X~2=9.26,P<0.01),该3个位点的单倍体型频率在UC组和对照组间差异亦有统计学意义(X~2=7.04,P<0.01).与对照组相比,UC组含等位基因型C者的血清IL-18含量明显升高(P<0.05).结论 IL-18基因的SNP与其血清含量存在相关性,提示其基因位点多态性及血清含量可能与UC易感性相关.

Association of the single nucleotide polymorphism and the serum contents of interleukine-18 with ulcerative colitis

Objective To estimate the association of the single nucleotide polymorphism (SNP) and the serum concentration of interleukin (IL)-18 with ulcerative colitis (UC) . Methods Samples collected from 50 patients with UC and 128 healthy controls were detected for SNPs of IL-18 gene including rs187238, rs5744228, rs360718 and rs360717 using polymerase chain reaction (PCR) and direct sequencing. Meanwhile, the serum level of IL-18 was measured. Results Serum concentration of IL-18 was higher in patients with UC than in healthy controls [(3029.9±111.7) pg/ml vs ( 133.2±39.4) pg/ml,(P<0.01)], rs187238 C, rs360718 C and rs360717 A were significantly related to UC (X~2=9.26, P<0.01). There was significant difference in frequencies of rs187238 C, rs360718 C and rs360717 A between patients with UC and healthy controls (X~2=9.26, P<0. 01). Serum concentration of IL-18 was higher in UC patients with C allele than in healthy controls (P<0.05).Conclusions There is correlation between SNP and serum level of IL-18 , which may involve in susceptibility to UC.