| 基于UHPLC-Q-TOF-MS分析荭草花提取物在H9c2心肌细胞中效应物质 |
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| 引用本文： | 向文英,梅朝叶,孙慧园,覃小丽,谢玉敏,巩仔鹏,郑林,李月婷,黄勇.基于UHPLC-Q-TOF-MS分析荭草花提取物在H9c2心肌细胞中效应物质[J].中国实验方剂学杂志,2017,23(12):90-95. |
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| 作者姓名： | 向文英 梅朝叶 孙慧园 覃小丽 谢玉敏 巩仔鹏 郑林 李月婷 黄勇 |
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| 作者单位： | 贵州医科大学 药学院, 贵州省药物制剂重点实验室, 国家苗药工程技术研究中心, 贵阳 550004,贵州医科大学 药学院, 贵州省药物制剂重点实验室, 国家苗药工程技术研究中心, 贵阳 550004,贵州医科大学 药学院, 贵州省药物制剂重点实验室, 国家苗药工程技术研究中心, 贵阳 550004,贵州医科大学 药学院, 贵州省药物制剂重点实验室, 国家苗药工程技术研究中心, 贵阳 550004,贵州医科大学 药学院, 贵州省药物制剂重点实验室, 国家苗药工程技术研究中心, 贵阳 550004,贵州医科大学 药学院, 贵州省药物制剂重点实验室, 国家苗药工程技术研究中心, 贵阳 550004,贵州医科大学 药学院, 贵州省药物制剂重点实验室, 国家苗药工程技术研究中心, 贵阳 550004,贵州医科大学 药学院, 贵州省药物制剂重点实验室, 国家苗药工程技术研究中心, 贵阳 550004,贵州医科大学 药学院, 贵州省药物制剂重点实验室, 国家苗药工程技术研究中心, 贵阳 550004 |
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| 基金项目： | 国家自然科学基金项目（81360664，81560630）；贵州省研究生卓越人才计划项目（黔教研合ZYRC字[2014]012） |
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| 摘 要： | 目的:利用H9c2心肌细胞和UHPLC-Q-TOF-MS技术筛选荭草花抗心肌缺血作用的活性成分。方法:用心肌细胞选择性地结合荭草花提取液中的活性成分,洗去未结合的其他成分后,使细胞靶点失活,被结合的活性成分从心肌细胞中释放出来,采用Eclipse Plus C_(18)色谱柱(2.1 mm×100 mm,1.8μm),流动相0.1%甲酸水溶液(A)-0.1%甲酸乙腈溶液(B)梯度洗脱(0~2 min,95%~79%A;2~5 min,79%A;5~6.5 min,79%~60%A;6.5~7.5 min,60%~55%A;7.5~9.5 min,55%~0%A;9.5~10 min,0%~95%A),电喷雾离子源(ESI)正、负离子模式下扫描采集数据,通过采用对照品比对及二级碎片离子对化学成分进行鉴定。结果:检测出荭草花提取物中可与心肌细胞结合的17个成分,采用对照品比对后,确定8个色谱峰所表征的化学成分,依次为原儿茶酸,儿茶素,山柰素-3-O-β-D-葡萄糖苷,槲皮素3-O-α-L-鼠李糖苷,山柰素-3-O-α-L-鼠李糖苷,N-p-香豆酰酪胺,槲皮素和山柰酚。结论:通过分析心肌细胞破碎液中与活性细胞相结合的成分,筛选出了荭草花提取物中能与心肌细胞有相互作用的成分,为明确荭草花的药效物质基础提供了科学依据。
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| 关 键 词： | UHPLC-Q-TOF-MS 荭草花提取物 活性成分 山柰素-3-O-β-D-葡萄糖苷 山柰素-3-O-α-L-鼠李糖苷 药效物质基础 |
| 收稿时间： | 2016/12/14 0:00:00 |
Identification of Effective Substances from Polygonum orientale Flower Extract in H9c2 Myocardial Cells by UHPLC-Q-TOF-MS |
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| XIANG Wen-ying,MEI Chao-ye,SUN Hui-yuan,QIN Xiao-li,XIE Yu-min,GONG Zi-peng,ZHENG Lin,LI Yue-ting and HUANG Yong.Identification of Effective Substances from Polygonum orientale Flower Extract in H9c2 Myocardial Cells by UHPLC-Q-TOF-MS[J].China Journal of Experimental Traditional Medical Formulae,2017,23(12):90-95. |
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| Authors: | XIANG Wen-ying MEI Chao-ye SUN Hui-yuan QIN Xiao-li XIE Yu-min GONG Zi-peng ZHENG Lin LI Yue-ting and HUANG Yong |
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| Institution: | National Engineering Research Center of Miao''s Medicines, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, School of Pharmacy, Guizhou Medical University, Guiyang 550004, China,National Engineering Research Center of Miao''s Medicines, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, School of Pharmacy, Guizhou Medical University, Guiyang 550004, China,National Engineering Research Center of Miao''s Medicines, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, School of Pharmacy, Guizhou Medical University, Guiyang 550004, China,National Engineering Research Center of Miao''s Medicines, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, School of Pharmacy, Guizhou Medical University, Guiyang 550004, China,National Engineering Research Center of Miao''s Medicines, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, School of Pharmacy, Guizhou Medical University, Guiyang 550004, China,National Engineering Research Center of Miao''s Medicines, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, School of Pharmacy, Guizhou Medical University, Guiyang 550004, China,National Engineering Research Center of Miao''s Medicines, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, School of Pharmacy, Guizhou Medical University, Guiyang 550004, China,National Engineering Research Center of Miao''s Medicines, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, School of Pharmacy, Guizhou Medical University, Guiyang 550004, China and National Engineering Research Center of Miao''s Medicines, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, School of Pharmacy, Guizhou Medical University, Guiyang 550004, China |
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