吉西他滨联合顺铂新辅助化疗对非小细胞肺癌TXNIP基因表达的影响

目的 研究吉西他滨联合顺铂新辅助化疗对非小细胞肺癌硫还蛋白结合蛋白氧(TXNIP)基因表达的影响。方法获取非小细胞肺癌(NSCLC)患者化疗前的肿瘤标本及采用吉西他滨联合顺铂(GP)方案新辅助化疗2个周期后的肿瘤标本,通过实时定量PCR法、免疫组化及Western blot分析标本中TXNIP表达的变化。并取一部分正常肺组织做为对照。结果正常人肺组织中TXNIP呈一定水平表达,相比之下,NSCLC患者癌标本中TXNIP信使RNA(mRNA)显著降低。当患者经2周期GP方案化疗后,癌标本中TXNIP mRNA表达水平明显增高；再次免疫组化及Western blot法检测结果显示,正常人群肺组织中TXNIP呈一定量的表达,而NSCLC患者癌标本中TXNIP表达量极低。但经GP方案治疗后,癌标本中TXNIP的含量增高。结论吉西他滨联合顺铂化疗能上调非小细胞肺癌TXNIP基因和蛋白的表达。

Effect of the neo-adjuvant chemotherapy of the combination of gemcitabine and cisplatin on txnip expression in non-small cell lung cancer cells

Objective To observe the effect of gemcitabine and cisplatin therapy on TXNIP expression in non-small cell lung cancer cells.MethodsThe healthy populations were collected,and non-small cell lung cancer patients were administrated with two course of gemcitabine and cisplatin,then the clinical specimens were collected before and after the treatment.The Thioredoxin-interacting protein (TXNIP) was detected by real time PCR,the protein were measured by immunohistochemistry and western blot.ResultThe mRNA of TXNIP in the healthy populations expressed to a certain level.In contrast,the TXNIP mRNA in NSCLC patients was lower than that in healthy control.After two courses of gemcitabine and cisplatin treatment,the TXNIP mRNA was gradually increased;Immunohistochemistry demonstrated that in the lung of the healthy population,TXNIP was expressed to a certain level,while it decreased in NSCLC patients.Gemcitabine and cisplatin administration could increase its expression level.ConclusionThe combination of gemcitabine and cisplatin can upregulate TXNIP gene and protein expression in non small cell lung cancer patients.