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Effect of nigranoic acid on Ca influx and its downstream signal mechanism in NGF-differentiated PC12 cells

Authors:	Xiao-Xi Yuan Li-Ping Yang Zhong-Lin Yang Wei-Lie Xiao Han-Dong Sun Gui-Sheng Wu Huai-Rong Luo

Institution:	1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China;2. University of Chinese Academy of Sciences, Beijing 100039, China;3. Yunnan Institute of Traditional Chinese Medicine and Materia Medica, Kunming 650223, Yunnan, China

Abstract:

Keywords:	SBB1 nigranoic acid AD Alzheimer?s disease PD Parkinson?s disease LTP long-term potentiation NO nitric oxide NOS NO synthase ERK1/2 extracellular signal regulated kinase 1 and 2 CaMKII calcium/calmodulin-dependent protein kinase II BDNF brain-derived neurotrophic factor MAPK mitogen-activated protein kinase CREB cyclic AMP-response element binging protein NGF nerve growth factor [Ca²⁺]_i intracellular Ca²⁺ concentration Schisandra chinensis Schisandra chinensis CNS central nervous system MTT 3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyl tetrazolium bromide DMSO dimethyl sulfoxide SDS sodium dodecyl sulfate EGTA ethylene glycol-bis (2-aminoethylether)-N N N&prime N&prime -tetraacetic acid BAPTA-AM 1 2-bis(2-amino-5-fluorophenoxy) ethane-N N N₂ N₂-tetraacetic acid tetrakis (acetoxymethyl) ester l-NAME N_ω-nitro-l-arginine methyl ester hydrochloride KN-62 1-[N O-bis(5-isoquinolinesulfonyl)-N-methyl-l-tyrosyl]-4-phenylpiperazine
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