| 参芍止带片的质量标准研究 |
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| 引用本文： | 南敏伦,;赫玉芳,;赵昱玮,;赵全成. 参芍止带片的质量标准研究[J]. 中国药房, 2014, 0(43): 4085-4087 |
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| 作者姓名： | 南敏伦, 赫玉芳, 赵昱玮, 赵全成 |
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| 作者单位： | [1]吉林省中医药科学院,长春130012; [2]吉林农业大学中药材学院,长春130118 |
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| 基金项目： | 吉林省科技发展计划项目（No.20120944） |
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| 摘 要： | 目的:建立参芍止带片的质量标准。方法:采用薄层色谱(TLC)法对制剂中的苦参、延胡索、香附、关黄柏进行定性鉴别;采用高效液相色谱法测定赤芍中芍药苷的含量:色谱柱为Agilent C18(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸溶液(18∶82,V/V),检测波长为230 nm,柱温为25℃,流速为1.0 ml/min。结果:苦参、延胡索、香附、关黄柏的TLC斑点清晰,分离度好。芍药苷的进样量在0.130 60.652 8μg范围内与其峰面积积分值呈良好线性关系(r=0.999 8);精密度、重复性、稳定性试验的RSD均≤1.97%;平均加样回收率为99.66%,RSD=1.09%(n=6)。结论:所建标准可用于参芍止带片的质量控制。
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| 关 键 词： | 参芍止带片 质量标准 薄层色谱法 高效液相色谱法 芍药苷 |
Study on Quality Standard for Shenshao Zhidai Tablets |
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| Affiliation: | NAN Min-lun, HE Yu-fang, ZHAO Yu-wei, ZHAO Quan-cheng (1.Jilin Province Academy of Chinese Medi- cal Sciences, Changchun 130012, China; 2.College of Chincse Medicinal Material, Jilin Agricultural University, Changchun 130118, China) |
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| Abstract: | OBJECTIVE： To establish the quality standard of Shenshao zhidai tablets. METHOD S：Sophora flavescens, Coryda- lis yanhusuo, Cyperus rotundus and Phellodendron amurense were identified by TLC qualitatively. The content of paeoniflorin was determined by HPLC. The separation was performed on Agilent-C18（250 mm×4.6 mm, 5 μm）column with mobile phase consisted of acetonitrile -0.1% phosphoric acid （18：82, V/V）at the flow rate of 1.0 ml/min. The detection wavelength was set at 230 nm, and the column temperature was 25℃. RESULTS： S. flavescens, C. yanhusuo, C. rotundus and P. amurense could be identified by TLC and separated well. The linear range of paeoniflorin were 0.130 6-0.652 8 μg（r=0.999 8）. RSDs of precision, stability and reproducibili- ty tests were all lower than 1.97%. The average recovery was 99.66% （RSD=1.09% ,n=6）. CONCLUSIONS： The established standard can be used for the quality control of Shenshao zhidai tablets. |
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| Keywords: | Shenshao zhidai tablets Quality standard TLC HPLC Paeoniflorin |
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