| 紫草中萘醌类化学成分的快速高效液相色谱-飞行时间质谱联用技术分析 |
| |
| 引用本文： | 毛艳,;张瑞萍,;贺金华,;贺玖明,;蔡晓翠,;严欢,;康雨彤.紫草中萘醌类化学成分的快速高效液相色谱-飞行时间质谱联用技术分析[J].中国药房,2014(43):4090-4092. |
| |
| 作者姓名： | 毛艳 ;张瑞萍 ;贺金华 ;贺玖明 ;蔡晓翠 ;严欢 ;康雨彤 |
| |
| 作者单位： | [1]新疆维吾尔自治区药物研究所,乌鲁木齐830004; [2]天然药物活性物质与功能国家重,点实验室/中国医学科学院北京协和医学院药物研究所,北京100050 |
| |
| 基金项目： | 新疆维吾尔自治区自然科学基金资助项目（No.201233146-8） |
| |
| 摘 要： | 目的:对紫草中萘醌类化学成分进行快速分离与鉴别。方法:采用快速高效液相色谱-飞行时间质谱(RRLC/Q-TOF/MS)法。色谱柱为Agilent SB-C18(100 mm×4.6 mm,1.8μm),流动相为乙腈-0.1%甲酸溶液(梯度洗脱),流速为0.4 ml/min,柱温为30℃;使用甲喷雾离子源,正、负离子扫描模式,载气温度为450℃,质量扫描范围(m/z)为1001 000。结果:推断出紫草中可能含有紫草素、β-羟基异戊酰紫草素、乙酰紫草素、β-乙酰氧基异戊酰紫草素、异丁酰紫草素、β,β-二甲基丙烯酰紫草素和α-甲基-正丁酰紫草素等10种化学成分。结论:该方法快速、高效,可为紫草的药效学和质量控制研究奠定基础。
|
| 关 键 词： | 紫草 萘醌 快速高效液相色谱-飞行时间质谱联用技术 |
Analysis of Naphthaquinones in Arnebia euchroma by RRLC/Q-TOF-MS |
| |
| Institution: | MAO Yan, ZHANG Rui-ping, HE Jin-hua, HE Jiu-ming, CAI Xiao-cui, YAN Huan, KANG Yu-tong ( 1.Xinjiang Institute of Materia Medica, Urumqi 830004, China; 2.State Key Laboratory of Bioactive Substance and Function of Natural Medicines/Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China) |
| |
| Abstract: | OBJECTIVE： To separate and identify naphthaquinones in Arnebia enchroma rapidly. METHODS： RRLC/Q-TOF/ MS was adopted. The separation was performed on Agilent SB-C18（ 100 minx4.6 mm, 1.8μm）column with mobile phase consisted of acetonitrile-0.1% formic acid （gradient elution） at the flow rate of 0.4 ml/min. The column temperature was 30 ℃. ESI was employed, and the sample was analyzed in the positive and negative-ion mode. The temperature of carrier gas was 450℃, mass scanning rang （m/z） was from 100-1 000. RESULTS： 10 kinds of naphthaquinones were identified by MS as sldkonin,fl-hydroxyisova- lerylshikonin, acetylshikonin, β-acetoxyisovalerylshikonin, isobutylshikonin, β,β-dimethylacrylshikonin and a-methybutyrylalkannietc, etc. CONCLUSIONS： The method is rapid and effective, and can provide experimental basis for pharmaeodynamics and quality control ofA. euchroma. |
| |
| Keywords: | Arnebia euchroma Naphthaquinones RRLC/Q-TOF/MS |
| 本文献已被 维普 等数据库收录！ |
|
