rhEPO对正常大鼠和链脲佐菌素诱导的急性糖尿病大鼠脑缺血的保护作用

目的:观察重组人促红细胞生成素(rhEPO)对正常大鼠与急性糖尿病大鼠局灶性脑缺血的神经功能、梗死灶体积的影响。方法:选择72只急性糖尿病大鼠(DR组)和54只正常大鼠(NR组),2组各平均分为假手术组、对照组和干预组3个小组,各小组按处死时间均分为第1天组、第7天组、第14天组。假手术组仅暴露颈内、外动脉,对照组和干预组均阻塞大脉中动脉,并分别腹腔注射等体积的生理盐水和rhEPO。采用Longa和神经功能缺损评分标准(NSS)评价神经功能,甲苯紫染色观察梗死灶体积变化。结果:NR组中,干预组与对照组比较,缺血再灌注后第1、7、14天的NSS改善分别为(4.17±4.88)(、2.67±3.01)(、1.83±1.72)(P均<0.05),DR组分别为(2.50±1.77)、(1.88±2.42)、(2.00±0.93)(P均<0.05),DR组与NR组比较差异无显著性意义。Longa评分,DR组对照组再灌注后第1天与NR组对照组差异无显著性意义(P>0.05),而第7、14天明显高于NR组对照组(P<0.05);NSS评分,DR组对照组再灌注后第1、7、14天均高于NR组对照组(P<0.05或0.01)。NR组干预组与对照组比较,缺血再灌注后第1、7、14天的梗死灶体积比分别减少(52%±7%)、(62%±7%)、(76%±3%)(P<0.05或0.01),DR组分别减少(50%±7%)、(57%±5%)、(69%±6%)(P<0.01),DR组与NR组比较差异无显著性意义(P>0.05)。DR组对照组缺血再灌注后第1、7、14天的梗死灶体积比非常明显高于NR组对照组(P<0.01)。结论:STZ诱导急性糖尿病合并脑缺血大鼠与正常脑缺血大鼠比较,功能缺损更重,梗死灶更大;rhE-PO能明显改善两者的神经功能并减小梗死灶,但对两者脑缺血的改善程度相似。

Neuroprotection of rhEPO on Cerebral Ischemia in both Normal and Pentylenetetrazol-induced Acute Diabetic Rats

Objective:To observe the influence of rhEPO on the neurological deficits,infarction territory proportion in focal cerebral ischemic rats with acute DM disease,and to compare these results with those of normal cerebral ischemic rats.Methods:The experimental animals experienced 1 hour of middle cerebral artery occlusion(MCAO) before reperfusion.A certain volume of rhEPO was injected at the same time of reperfusion.Longa and NSS tests were performed to value neurological function and the infarction territories were shown through crystal-violet staining.Results:In the normal rats,rhEPO can significantly improve the NSS by(4.17±4.88)、(2.67±3.01)、(1.83±1.72) respectively(P<0.05);while in the acute DM ones,rhEPO can significantly improve them by(2.50±1.77)、(1.88±2.42)、(2.00±0.93) respectively as well (P<0.05).At the 1st day after reperfusion,there was no difference between the Longa of DM control group and that of normal control((P>)0.05),while the Longa at the 7th,14th day after reperfusion of DM control group were significantly higher than those of normal control(P<0.05);At the 1st,7th,14th day after reperfusion,the NSS of DM control group were all significantly higher than those of normal control(P<0.01).In the normal rats,the treatment of rhEPO can reduce infarction territory at the different three time points by(52%±7%)、(62%±7%)、(76%±3%) respectively(P<0.05 or 0.01),while in the acute DM ones,the reduction of infarction were(50%±7%)、(57%±5%)、(69%±6%) respectively(P<0.01).The changes in the acute DM rats were of no significance when referred to those in the normal(P>0.05).At the 1st,7th,14th day after reperfusion,the proportion of infarction part of the DM control group was significantly larger than those of normal control((P<)0.01). Conclusion: rhEPO can significantly ameliorate the neurological deficit and reduce the infarction area in the rats with focal cerebral ischemia in both groups with a similar pattern.The neurological deficits of focal cerebral ischemic rats with acute STZ-induced DM were significantly severer than those of the normal ones.