| 鼠抗丙型肝炎病毒ns－5区单克隆抗体的研究 |
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| 引用本文： | 刘莹,李秀华,张宁,孟淑芳,尹红章,李德富.鼠抗丙型肝炎病毒ns－5区单克隆抗体的研究[J].细胞与分子免疫学杂志,1995(2). |
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| 作者姓名： | 刘莹 李秀华 张宁 孟淑芳 尹红章 李德富 |
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| 作者单位： | 中国药品生物制品检定所 |
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| 摘 要： | 用ＨＣＶ非结构区ｎｓ－５合成多肽抗原交联后免疫小鼠，成功地建立了３株抗ｎｓ－５单克隆抗体（ＭｃＡｂ），经检测这３株ＭｃＡｂ属于同一位点，与其它区域无明显交叉反应，具有高度特异性。ＭｃＡｂ的研制为检测ｎｓ－５抗原奠定了一定基础。
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| 关 键 词： | HCV 单克隆抗体 ns－5抗原 抗原检测 |
Preporation of Mouse Monoclonal Antibody against HCV, ns-5 |
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| Liu Ying,et al.Preporation of Mouse Monoclonal Antibody against HCV, ns-5[J].Journal of Cellular and Molecular Immunology,1995(2). |
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| Authors: | Liu Ying |
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| Abstract: | Three hybridomas(McAb ns-5)secreting monoclonal antibodies against ns-5 were prepared by fusing Sp2/0 with spleen cells from BALB/c mice immunized with ns-5 antigen.Competitiveinhibition test using ELISA showed the three monoclonal antibodies bearing a same binding site to ns-5with no cross reaction with other HCV antigens. The study provides a useful implementation for detection of ns-5 antigen. |
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| Keywords: | HCV antibody monoclonal ns-5 antigen antigen detection |
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