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| 槲皮素对大鼠原代肝细胞HO-1蛋白表达及活性的影响 | |
| 引用本文： | 刘爽,姚平,龚晨睿,李骏,程茅伟,刘烈刚. 槲皮素对大鼠原代肝细胞HO-1蛋白表达及活性的影响[J]. 营养学报, 2011, 33(6) |
| 作者姓名： | 刘爽 姚平 龚晨睿 李骏 程茅伟 刘烈刚 |
| 作者单位： | 1. 华中科技大学同济医学院公共卫生学院营养与食品卫生学系,武汉430030;湖北省疾病预防控制中心,武汉430079 2. 华中科技大学同济医学院公共卫生学院营养与食品卫生学系,武汉,430030 3. 湖北省疾病预防控制中心,武汉,430079 |
| 基金项目： | 国家自然科学基金青年科学基金课题(No.30500405) |
| 摘 要： | 目的研究槲皮素对大鼠原代肝细胞中I型血红素氧化酶(HO-1)蛋白表达及酶活性的影响。方法二步胶原酶技术分离培养大鼠原代肝细胞。用不同剂量(25、50、100、200 mol/L)槲皮素作用大鼠原代肝细胞12 h,或用50 mol/L槲皮素作用肝细胞不同时间(2～12 h)后,提取肝细胞微粒体,检测HO-1酶活性变化,并用WesternBlot方法检测HO-1蛋白表达水平的变化。结果大鼠原代肝细胞经50～200 mol/L槲皮素处理12 h,或用50 mol/L槲皮素处理4～12 h后,HO-1蛋白表达水平和酶活性与对照组比较明显升高(P<0.05)。结论槲皮素能够诱导大鼠原代肝细胞的HO-1蛋白表达,提高肝细胞内HO-1的酶活性。 |
| 关 键 词： | 槲皮素 肝细胞 Ⅰ型血红素氧化酶 |
EFFECTS OF QUERCETIN ON HEME OXYGENASE-1 PROTEIN EXPRESSION AND ENZYME ACTIVITY IN RAT PRIMARY HEPATOCYTES |
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| LIU Shuang , YAO Ping , GONG Chen-rui , LI Jun , CHENG Mao-wei , LIU Lie-gang. EFFECTS OF QUERCETIN ON HEME OXYGENASE-1 PROTEIN EXPRESSION AND ENZYME ACTIVITY IN RAT PRIMARY HEPATOCYTES[J]. Acta Nutrimenta Sinica, 2011, 33(6) | |
| Authors: | LIU Shuang YAO Ping GONG Chen-rui LI Jun CHENG Mao-wei LIU Lie-gang |
| Affiliation: | LIU Shuang,YAO Ping,GONG Chen-rui,LI Jun,CHENG Mao-wei,LIU Lie-gang(1Department of Nutrition and Food Hygiene,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,2Hubei Center for Disease Control and Prevention,Wuhan 430079,China) |
| Abstract: | Objective To investigate the effects of quercetin on heme oxygenase-1(HO-1) protein expression and enzyme activity in rat primary hepatocytes.Method Sprague-Dawley rat primary hepatocytes were isolated using two-step collagenase perfusion technique.The hepatocytes were treated with quercetin of different doses(25-200 μmol/L) for 12 h,or were treated with 50 μmol/L quercetin for different time(4-12 h).Then Western blot and enzymatic assays were deployded to determine the protein expression and the enzyme activity of HO-1 in hepatocellular microsomes.Results After co-incubation with quercetin at the dose of 50-200 μmol/L for 4-12 h,the protein level and the enzyme activity of HO-1 in hepatocytes increased significantly compared with the control group(P<0.05).Conclusion Quercetin could up-regulate HO-1 protein expression and improve HO-1 enzyme activity in rat primary hepatocytes. |
| Keywords: | quercetin hepatocyte heme oxygenase-1 |
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