血浆非特异性免疫抑制蛋白对大鼠淋巴细胞粘附主动脉内皮细胞的影响

淋巴细胞粘附于血管内皮细胞(EC)是它们从血液迁移进入血管外组织的关键之一。我们利用主动脉内膜制备(Hautchen)技术和免疫荧光标记法初步研究了猪血浆非特异性免疫抑制蛋白(PNIP)对淋巴细胞粘附主动脉EC的影响,结果表明,PNIP不仅能抑制淋巴细胞的丝裂原反应而且也能够显著地抑制血液、胸腺或肠系膜淋巴结(MLN)淋巴细胞粘附主动脉EC。研究还发现,粘附细胞中膜表面免疫球蛋白IgG阳性(SmIgG~ )B细胞所占比例明显下降。这些结果提示血浆免疫抑制蛋白能够抑制淋巴细胞对EC的粘附,从而可能影响炎症,动脉粥样硬化和血栓的形成。

Effects of plasma non-specific immunosuppressive protein (PNIP) on adhesion of lymphocytes to aortic endothelial cells (EC) in rats

Adhesion of lymphocytes to EC is one of the important steps of lympho. cyte migration into the tissues outside vessels; and the components of blood plasma may have some effect on the adhesion of lymphocytes. In our experiments, we observed that PNIP, normally present in blood and being able to inhibit the proliferation of lymphocytes stimulated by antigens or mitogens, could significantly inhibit the adhesion of the lymphocytes from blood, thymus and mesenteric lymph nodes (MLN) to the aortic EC, using the aortic EC monolayer preparation (Hautchen technique). Meanwhile, the indirect immunofluorescent test also showed us that PNIP might result in a remarkable decrease in percentage of SmIgG positive B lymphocytes in the total lymphocytes adhering to the aortic EC. These results imply that PNIP may suppress the adhesion of lymphocytes or other leukocytes to EC in the development of inflammation, atherosclerosis or thrombosis.