Identification and functional characterization of a solute carrier family 15, member 4 gene in Litopenaeus vannamei |
| |
| Affiliation: | 1. Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering/School of Marine Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China;2. School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China;3. State Key Laboratory for Biocontrol/MOE Key Laboratory of Aquatic Product Safety/Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China;1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China;2. University of Chinese Academy of Sciences, Beijing, 100049, China;3. National & Local Joint Engineering Laboratory of Ecological Mariculture, 7 Nanhai Road, Qingdao, 266071, China;1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China;2. Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, 116023, China;3. University of Chinese Academy of Sciences, Beijing, 100049, China;1. College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China;2. Ministry of Education and Shanghai Key Laboratory of Children''s Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China;3. School of Resource & Environmental Management, Guizhou University of Finance and Economics, Guizhou 550025, China;1. Department of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, Ann Arbor, MI, USA;2. Department of Neurosurgery, University of Michigan Health System, Ann Arbor, MI, USA;3. Department of Gastroenterology, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan |
| |
| Abstract: | Innate immunity in shrimp is important in resisting bacterial infection. The NF-κB pathway is pivotal in such an immune response. This study cloned and functionally characterized the solute carrier family (SLC) 15 member A 4 (LvSLC15A4) gene in Litopenaeus vannamei. The open reading frame of LvSLC15A4 is 1, 902 bp long and encodes a putative 633-amino acid protein, which is localized in the plasma membrane and intracellular vesicular compartments. Results of the reporter gene assay showed that LvSLC15A4 upregulated NF-κB target genes, including the immediate-early gene 1 of white spot syndrome virus, as well as several antimicrobial peptide genes, such as pen4, CecA, AttA, and Mtk in S2 cells. Moreover, knocked-down expression of LvSLC15A4 reduced pen4 expression in L. vannamei. LvSLC15A4 down-regulation also increased the cumulative mortality of Vibrio parahemolyticus-infected L. vannamei. Furthermore, LvSLC15A4 expression was induced by unfolded protein response (UPR) in L. vannamei hematocytes. These results suggest that LvSLC15A4 participates in L. vannamei innate immunity via the NF-κB pathway and thus may be related to UPR. |
| |
| Keywords: | Toll-like recepter NF-κB Solute carrier family 15 member A 4 Unfolded protein response |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
