应用等位基因特异性PCR检测血管紧张素原基因突变

目的:建立检测血管紧张素原(angiotensinogen,AGT)基因2种常见突变的等位基因特异性PCR技术。方法:应用针对AGT基因T174M和M235T这2种常见的突变位点设计的等位基因特异性PCR技术,对海南汉、黎族人群中AGT基因突变类型进行了检测,同时对经上述等位基因特异性PCR检测的样本进行序列测定。结果:在海南汉、黎族人群中,T174M突变位点可检测出TT、TM、MM 3种基因型,M235T突变位点可检测出MM、MT、TT 3种基因型,用等位基因特异性PCR鉴定的AGT基因突变的基因分型结果与序列测定结果完全符合。结论:等位基因特异性PCR技术操作简便,重复性和稳定性好,可作为鉴定AGT基因突变类型的可行方法。

Detection of the AGT gene mutation by AS-POR

Objective： To establish the allele specific polymerase chain reaction（AS-PCR） technique for the detection of 2 normal mutations of angiotensinogen（AGT） gene.Methods： Designed AS-PCR system was used to detect the mutations in Li and Han nationalities of Hainan province towards 2 common mutations of AGT gene,T174M and M235T.The sequence of the AS-PCR sample was detected simultaneously.Results： Three genotypes were successfully detected in T174M mutation site which were TT,TM and MM,and 3 genotypes were successfully detected in M235T mutation site which were MM,MT and TT.All the results above provided by the AS-PCR were exactly consistent with the sequencing results.Conclusion： The AS-PCR with advantages of stability and simplify is an effective method for investigation of the mutations of AGT gene.