中西医结合疗法对暴露性干眼症大鼠的干预研究

目的：探讨中西医结合疗法对暴露性干眼症大鼠的干预作用及其机制。方法：将100只（200只眼）健康Wistar大鼠随机分为正常对照组（A组）、模型对照组（B组）、贝复舒组（c组）、贝复舒加中药凉雾组（D组），每组25只（50只眼）。B、C、D组通过机械性阻止大鼠瞬目，暴露角结膜制作干眼症模型？造模成功后，A组生理盐水点眼，B组不给予任何处理，C组给予贝复舒眼液点眼，D组给予贝复舒眼液点眼，同时给予中药凉雾治疗。各组分别于给药后第l、3、5、7、14天进行SchirmerI试验、荧光素染色检查，并处死大鼠取角膜进行病理学检查。结果：SchirmerI试验痣纸湿润长度比较，C组第14天、D组第7、14天与A组比较，差异均无统计学意义（P〉0．05）；第1、3、5、7、14天C、D组与B组比较，滤纸湿润长度均增加，差异均有统计学意义（P〈0．01）。角膜荧光素染色试验染色点评分，第3、5、7、14天C、D组与B组比较，染色点减少，差异均有统计学意义（P〈0．01）。角膜病理变化比较，第5天开始D组角膜上皮基本光滑，结构接近正常组；第7天，C组角膜结构基本同正常组；第14天B组角膜基本修复，但不光滑．结论：中西医结合疗法可使暴露性干眼症模型大鼠泪液分泌增多，染色点减少，恢复受损的角膜上皮细胞、

Therapeutic effect of integrated traditional Chinese and Western medicine therapy in rats with xerophthalmia due to corneal and conjunctival exposure

Objeetive：To investigate the therapeutic effect of integrated traditional Chinese and Westenq medicine therapy in rats with xerophthalmia due to eorneal and conjunctival exposure and its mechanism. Methods：A total of 100 healthy Wistar rats （with 200 eyes） were randnmly divided into normal control group （group A）, model control group （group B ）, recombinant hovine basic fibroblast growth factor （rb -bFGF） group （group C） ,and rb -bFGF plus cool atomized traditional Chinese medicines group （group D） ,with 25 rats （50 eyes） in each group. The rats in groups B,C ,and D were mechanically stopped from blinking to expose the cornea and conjunetiva and establish a model of xerophthahnia. Atier the model was successfully established,group A was given eye drops of normal saline, group B received no treatment,group C was given eye drops of rb -bFGF,and group D was given eye drops of rb -bFGF and cool atomized tradi- tional Chinese medieines. Each group underwent Schirmer I test and fluorescein staining at 1,3,5,7 ,and 14 d after treatment. Then, all rats were sacrificed to collect the eornea for pathological examination. Results：The Schirmer I test showed that the lengths of wet filter pa- per in group C at 14 d and group D at 7 and 14 d were not significantly different tYom that in group A （P 〉0.05） ;groups C and D had significantly inereased lengths of wet filter paper compared with group B at 1,3,5,7 ,and 14 d （ P 〈0. 01 ）. The fluoreseein staining of the cornea showed that groups C and D had significantly reduced stained dots compared with group B at 3,5,7, and 14 d （ P 〈 0.01 ）. The pathological examination showed that the corneal epithelium in group D became basically smooth with an approximately normal structure at 5 d;group C had almost the same corneal structure as group A at 7 d;the cornea in group B was nearly repaired but not smooth at 14 d. Conclusion：Integrated traditional Chinese and Western medicine therapy can increase tear secretion,reduee stained dots,and repair the in- jured corneal epithelium in rats with xerophthalmia due to corneal and conjunctival exposure.