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Prolactin (PRL) induction of cyclooxygenase 2 (COX2) expression and prostaglandin (PG) production in hamster Leydig cells |
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| Authors: | Matzkin María Eugenia Ambao Verónica Carino Mónica Herminia Rossi Soledad Paola González Lorena Turyn Daniel Campo Stella Calandra Ricardo Saúl Frungieri Mónica Beatriz |
| Affiliation: | a Laboratorio de Esteroides, Instituto de Biología y Medicina Experimental, CONICET, Vuelta de Obligado 2490 (1428), Ciudad de Buenos Aires, Argentina b Departamento de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155 (1121), Ciudad de Buenos Aires, Argentina c Centro de Investigaciones Endocrinológicas, Hospital General de Niños R. Gutiérrez, CONICET, Gallo 1330 (1425), Ciudad de Buenos Aires, Argentina d Cátedra de Histología-Embriología B, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, CICPBA, Calles 60 y 120 (1900), La Plata, Argentina e Departamento de Química Biológica, Instituto de Química y Fisicoquímica Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires-CONICET, Junín 956 (1113), Ciudad de Buenos Aires, Argentina |
| Abstract: | Serum prolactin (PRL) variations play a crucial role in the photoperiodic-induced testicular regression-recrudescence transition in hamsters. We have previously shown that cyclooxygenase 2 (COX2), a key enzyme in the biosynthesis of prostaglandins (PGs), is expressed mostly in Leydig cells of reproductively active hamsters with considerable circulating and pituitary levels of PRL. In this study, we describe a stimulatory effect of PRL on COX2/PGs in hamster Leydig cells, which is mediated by IL-1β and prevented by P38-MAPK and JAK2 inhibitors. Furthermore, by preparative isoelectric focusing (IEF), we isolated PRL charge analogues from pituitaries of active [isoelectric points (pI): 5.16, 4.61, and 4.34] and regressed (pI: 5.44) hamsters. More acidic PRL charge analogues strongly induced COX2 expression, while less acidic ones had no effect.Our studies suggest that PRL induces COX2/PGs in hamster Leydig cells through IL-1β and activation of P38-MAPK and JAK2. PRL microheterogeneity detected in active/inactive hamsters may be responsible for the photoperiodic variations of COX2 expression in Leydig cells. |
| Keywords: | Prolactin Cyclooxygenase 2 Prostaglandins Leydig cells Hamsters |
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