| 柱前衍生化HPLC法建立苦豆子多糖特征图谱的研究 |
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| 引用本文： | 张岩,赵振宇,王苹,张桂贤,傅予,刘庆焕,陶遵威.柱前衍生化HPLC法建立苦豆子多糖特征图谱的研究[J].天津中医药,2017,34(9):634-637. |
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| 作者姓名： | 张岩 赵振宇 王苹 张桂贤 傅予 刘庆焕 陶遵威 |
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| 作者单位： | 天津市医药科学研究所, 天津 300020,天津市中医药研究院附属医院, 天津 300120,天士力控股集团, 天津 300410,天津市医药科学研究所, 天津 300020,天津市医药科学研究所, 天津 300020,天津市医药科学研究所, 天津 300020,天津市医药科学研究所, 天津 300020 |
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| 基金项目： | 天津市科技计划项目（12ZCDZSY19200）。 |
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| 摘 要： | 目的]建立苦豆子多糖特征图谱。方法]采用1-苯基-3-甲基本5-吡唑酮(PMP)柱前衍生化高效液相色谱(HPLC)法对苦豆子多糖进行单糖组成分析。结果]苦豆子多糖特征图谱由7个共有峰组成,为甘露糖、葡萄糖、鼠李糖、半乳糖、木糖、阿拉伯糖以及1个未鉴定出的单糖。结论]柱前衍生化HPLC法建立多糖的特征图谱操作简便、准确、重复性良好,为苦豆子多糖的质量评价提供了依据。
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| 关 键 词： | 苦豆子多糖 柱前衍生化 HPLC 特征图谱 单糖 |
| 收稿时间： | 2017/3/25 0:00:00 |
Study on establishing specific chromatogram of Sophora alopecuroides L. polysaccharides by pre-column derivation HPLC method |
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| ZHANG Yan,ZHAO Zhen-yu,WANG Ping,ZHANG Gui-xian,FU Yu,LIU Qing-Huan and TAO Zun-wei.Study on establishing specific chromatogram of Sophora alopecuroides L. polysaccharides by pre-column derivation HPLC method[J].Tianjin Journal of Traditional Chin Medicine,2017,34(9):634-637. |
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| Authors: | ZHANG Yan ZHAO Zhen-yu WANG Ping ZHANG Gui-xian FU Yu LIU Qing-Huan and TAO Zun-wei |
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| Institution: | Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China,Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin 300120, China,Tasly Holding Group, Tianjin 300410, China,Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China,Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China,Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China and Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China |
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| Abstract: | Objective] To establish specific chromatogram of Sophora alopecuroides L. polysaccharides.Methods] The monosaccharide composition of Sophora alopecuroides L. polysaccharides was determined by pre-column derivation HPLC method.Results] There were 7 peaks from the fingerprint which were mannose, glucose, rhamnose, galactose, xylose arabinose and an unidentifiable monosaccharide respectively.Conclusion] The pre-column derivation HPLC method establishing specific chromatogram is a simple, accurate and reproducible. And the study can provide evidence for quality evaluation of Sophora alopecuroides L. polysaccharides. |
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| Keywords: | Sophora alopecuroides L polysaccharides pre-column derivation HPLC specific chromatogram monosaccharide |
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