| Determination of amitriptyline and nortriptyline in human liver microsomes with reversed-phase HPLC in vitro |
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| 作者姓名： | 舒焱 朱荣华 许振华 周宏灏 |
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| 作者单位： | 湖南医科大学遗传药理学研究所 |
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| 摘 要： | ＤｅｔｅｒｍｉｎａｔｉｏｎｏｆａｍｉｔｒｉｐｔｙｌｉｎｅａｎｄｎｏｒｔｒｉｐｔｙｌｉｎｅｉｎｈｕｍａｎｌｉｖｅｒｍｉｃｒｏｓｏｍｅｓｗｉｔｈｒｅｖｅｒｓｅｄｐｈａｓｅＨＰＬＣｉｎｖｉｔｒｏ１ＳＨＵＹａｎ，ＺＨＵＲｏｎｇＨｕａ２，ＸＵＺｈｅｎＨｕ...
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| 关 键 词： | 阿米替林 去甲替林 高压液相色谱法 肝微粒体 |
Determination of amitriptyline and nortriptyline in human liver microsomes with reversed phase HPLC in vitro 1 |
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| SHU Yan,ZHU Rong Hua ,XU Zhen Hua,ZHOU Hong Hao.Determination of amitriptyline and nortriptyline in human liver microsomes with reversed-phase HPLC in vitro[J].Acta Pharmacologica Sinica,1998(4). |
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| Authors: | SHU Yan ZHU Rong Hua XU Zhen Hua ZHOU Hong Hao |
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| Institution: | SHU Yan,ZHU Rong Hua 2,XU Zhen Hua,ZHOU Hong Hao 3 |
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| Abstract: | AIM: To develop a method for simultaneous determinations of amitriptyline (Ami) and its metabolite nortriptyline (Nor) in human liver microsomes. METHODS: An incubation buffer containing microsomes, NADPH generating system, and Ami, after termination of enzyme reaction and desipramine (Des) as internal standard (IS), was extracted with diethy ether and separated on a reversed phase ODS column. Detection was achieved at 242 nm by ultraviolet detector. RESULTS: No potential interfering peaks were found. Ami and Nor gave rapid elution and baseline resolution. The linear curves of both analyses ranged 0 02-10 nmol and the limit of detection was 0 01 nmol. The recovery (94 %-101 %) had good precision with relative s of <8 3 % . CONCLUSION: This method is rapid, sensitive, and simple for studying the metabolism of Ami and Nor. |
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| Keywords: | amitriptyline nortriptyline high pressure liquid chromatography liver microsomes |
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