蛋白激酶C-α、CyclinD1和Cdk4在大肠癌中表达的相关性及临床意义

目的 探讨PKC α,CyclinD1和Cdk4在大肠癌中表达的相关性及其与临床病理学特征的关系。方法 应用免疫组化SP法对83例大肠组织中PKC α,CyclinD1和Cdk4的表达进行检测。结果 PKC α在大肠癌组织中阳性表达31/47（66.0%）与正常组织3/15（20.0%）及腺瘤10/21（47.6%）相比差异有统计学意义（P<0.05），与大肠癌分化程度，Dukes分期，淋巴结转移及CEA水平明显相关（P<0.05）。CyclinD1和Cdk4在大肠癌中阳性表达分别为26/47（55.5%）、28/47（59.6%），与正常组织中2/15（13.3%）、1/15（6.70%）及腺瘤组织中8/21(38.1%)、9/21（42.9%）相比呈递增趋势（P<0.05）。大肠癌中CyclinD1阳性表达与肿瘤分化程度，Dukes分期，淋巴结转移呈正相关（P<0.05），而与CEA水平关系不大（P＞0.05）；Cdk4阳性表达则与分化程度，Dukes分期有关（P<0.05），与淋巴结转移及CEA水平关系不密切（P＞0.05）。PKC α与CyclinD1呈正相关关系（r=0.348,P<0.05），CyclinD1和Cdk4呈正相关关系（r=0.393,P<0.05），PKC α和Cdk4则无明显相关性（r=0.167,P＞0.05）。结论 蛋白激酶C α与CyclinD1及Cdk4的过表达在大肠癌的发生、发展中起着重要的协同作用。三者的过表达与大肠癌的分化程度，Dukes分期密切相关。

Expression of Protein Kinase C alpha,CyclinD1 and Cdk4 Proteins in Colorectal Carcinomas and Their Clinical Significance

Objective 　To investigate the relationship between the expression of PKC2α、CyclinD1 and Cdk4 and the clinical significance in colorectal carcinomas.Methods 　Immunohistochemical staining was used to detect the expressions of PKC2α,CyclinD1 and Cdk4 in normal colorectal tissues , adenoma and carcinoma , respectively. Results 　The Positive rates of PKC2α expression in normal colorectal tissues , adenoma and carcinomas were 20 %, 47 %, 66. 0 %, respectively ( P < 0. 05) , associated with the tumor cellular differentiation ,Dukes phase and lymph node metastasis and CEA , respectively ( P < 0. 05) . Overexpression of CyclinD1 and Cdk4 were 55. 3 %,59. 6 % in colorectal carcinomas , showed an increasing tendency in colorectal carcinogenesis , respective2 ly ( P < 0. 05) ; the expression of CyclinD1 was correlated closely with differentiated degree , Dukes phage , lymph node metastasis and CEA , respectively ( P < 0. 05) ;the expression of Cdk4 was associated with differen2 tiated degree , Dukes phase and CEA , respectively ( P < 0. 05) . There is a positive relationship between PKC2α and CyclinD1 ( r = 0. 348 , P < 0. 05) , There is a positive relationship between CyclinD1 and Cdk4 ( r = 0. 393 , P < 0. 05) . Conclusion 　Overexpression of PKC2α、CyclinD1 and Cdk4 may cont ribute to the pathogenesis of colorectal carcinoma ,and it was correlated closely with differentiated degree , Dukes phase and CEA.