c-erbB-2多克隆抗体的制备及特性分析

目的　制备针对表皮生长因子受体 (c erbB 2 )细胞表面受体的抗体 ,不仅可阻止生长信号的传递 ,从而抑制肿瘤生长 ,还可通过抗体介导的细胞毒作用杀伤肿瘤细胞 ,具有重要的临床治疗应用价值。方法　通过重组表达获得了纯化的c erbB 2基因产物———融合蛋白GST c erbB 2 ,用此抗原制备兔抗血清 ,经rProteinGAgarose和GlutathioneSepharose 4B亲和层析柱纯化获得IgG型抗c erbB 2蛋白的多克隆抗体。对此抗体进行了细胞表面特异识别、体外肿瘤细胞生长抑制等方面的功能研究。结果　抗体可特异性识别重组表达蛋白、细胞表面c erbB 2抗原 ,并与之结合 ;抗体对c erbB 2阳性的人乳腺癌细胞SK BR 3有特异性的杀伤作用 ,且呈剂量相关 ,而对c erbB 2阴性的人乳腺癌细胞MCF 7则无明显作用。结论　c erbB 2抗体通过其对c erbB 2阳性细胞的特异识别和生长抑制 ,有广阔的临床应用前景。

Preparation of Anti-cerbB-2 Polyclonal Antibody and Its Effects on Human Breast Cancer Cells

Purpose c-erbB?2 is an attractive target for the delivery of therapeutic agents.Preparing antibody binding the ligand of c-erbB?2 can not only block the pathway of growth factors but also induce antibody dependent cell-mediated cytotoxity. Methods c-erbB?2 was highly expressed in E.coli BL21(DE3) and purified.The c-erbB?2 protein was used as antigen to get IgG polyclonal antibody.The specificity and efficiency of the antibody were analyzed. Results The antibody thus prepared can inhibit growth of SK?BR?3 cells lines,which are c-erbB?2 positive; but has no effect on MCF?7 cell lines,which are c-erbB?2 negative. Conclusions The c-erbB?2 antibody may be beneficial to cancer therapy by specific recognization and tumor cells growth inhibition.