环磷酰胺诱导少精子/无精子症大鼠模型所致睾丸、附睾IGF-I的变化

目的:探讨环磷酰胺(CP)对大鼠附睾和睾丸组织中胰岛素样生长因子I(IGF-I)表达的影响;方法:96只8周龄成年雄性SD大鼠,分为6组,每组16只。第1~5组分别给予CP 10、20、40、80、100 mg/(kg.d),第6组为对照组,给予生理盐水2 m l。胃管给药2周和4周后,处死大鼠,采用ELISA法对附睾组织洗脱液IGF-I进行定量检测;同时采用SP免疫组化法检测睾丸组织IGF-I的表达。结果:随着CP处理时间的延长和剂量的增加,IGF-I在大鼠附睾中的浓度逐渐下降,大鼠睾丸组织中IGF-I的表达也降低。结论:CP在引起大鼠少精子/无精子症的同时,可以显著降低大鼠附睾、睾丸IGF-I的含量和表达水平。IGF-I的降低与CP降低大鼠生精功能明显相关。

The Changes of IGF-Ⅰ in Testis and Epididymis on a Rat Model with Oligoazoospermia/azoospermia Induced by Cyclophosphamide

Objective: To evaluate the effect of the levels of IGF-Ⅰin the epididymis and the expression of IGF-Ⅰ in the testis of adult male rat after the administration of cyclophosphamide.Methods: Ninety-six male adult rats(8 weeks age)were divided into 6 groups.The doses given to the rats of the groups 1 to 5 were 10,20,40,80 and 100 mg/(kg·d),respectively.The remaining group was served as control.All those rats were sacrificed and IGF-I were quantitatively determined by ELISA techniques 2 and 4 weeks after the administration of the drug(by gastric fudge).Immunohistochemical SP technique was used to examine expression of IGF-I in rat testis. Results: The levels of cell factors(IGF-I)in the epididymis of the rats were gradually reduced with the increasing time and dose after administration of the drug.In the mean time the expression of IGF-I in the tissues of the testis of those rats were also gradually reduced.Conclusion: In the time of oligozoospermia/azoospermia induced by the administration of cyclophosphamide,the expression levels of IGF-I in the genetic system were significantly reduced.The possible mechanism of these changes could be attributed to the lower spermatogeneous function of the testis caused by the administration of cyclophosphamide.