大肠癌PKC-α、CyclinD1和Cdk4的表达与临床病理学特征的关系

目的：探讨大肠癌PKC-α，CyclinD1和Cdk4的表达与临床病理学特征的关系。方法：应用免疫组织化学sP法对PKC-α，CyclinD1和Cdk4的表达进行检测。结果：PKC-α在大肠癌组织中阳性表达率31／47（66．0％）与大肠正常组织3／15（20．0％）及腺瘤10／21（47．6％）对比有显著性差异（P〈0．05），与大肠癌分化程度，Dukes分期，淋巴结转移及CEA水平明显相关（P〈0．05）。CyclinD1和Cdk4在大肠癌中阳性表达率分别为26／47（55．5％），28／47（59．6％），与正常组织中表达2／15（13．3％），1／15（6．70％）及腺瘤组织中表达8／21（38．1％），9／21（42．9％）相比呈递增趋势（P〈0．05）。大肠癌中CyclinD1阳性表达与肿瘤分化程度，Dukes分期，淋巴结转移呈正相关关系（P〈0．05），而与CEA水平关系不大（P〉0．05）；Cdk4阳性表达则与分化程度，Dukes分期有关（P〈0．05），与淋巴结转移及CEA水平关系不密切（P〉0．05）。结论：PKC-α，Cy-clinD1和Cdk4的高表达与大肠癌分化程度，Dukes分期呈明显相关，对判断大肠肿瘤恶性程度具有一定的临床意义。

Relationship between the expression of PKC-α,CyclinD1, Cdk4 and clinicopathological characteristics in human colorectal carcinoma

Objective：To investigate the relationship between the expression of PKC -α,CyclinD1 , Cdk4 and the clinical pathological characteristics in colorectal carcinomas. Methods： Immunohistochemlcal staining was used to detect the expressions of PKC-α, CyclinDl and Cdk4 in the normal colorectal tissues, adenomas and carcinomas, respectively. Results： The positive rates of PKC-α expression in normal colorectal tissues, adenomas and carcinomas were 20% , 47% , 66.0%, respectively（ P 〈 0.05 ）, associated with the tumor cellular differentiation , Dukes＇ phase and lymph node metastasis and CEA （P 〈 0.05 ）. Overexpression of CyclinDl and Cdk4 were 55.3% , 59.6% in the colorectal carcinomas, showed an increasing tendency in colorectal carcinogenesis （ P 〈 0.05） ; The expression of CyclinDl was correlated closely with differentiated degree, Dukes phage , lymph node metastasis and CEA （P 〈0.05） ;The expression of Cdk4 was associated with differentiated degree ,Dukes phase and CEA （P 〈0.05）. Conclusion： Overexpression of PKC -α, CyclinD1 and Cdk4 in colorectal carcinomas was correlated closely with differen- tiated degree, Dukes phase and may contribute to the pathogenesis of colorectal carcinoma.