食蟹猴急性酒精性脂肪肝脾胃湿热证模型的建立与评价初探

目的:建立急性酒精性脂肪肝脾胃湿热证食蟹猴模型,为酒精性脂肪肝的中医药现代研究提供研究工具。方法:将6只健康成年雄性食蟹猴按体质量随机分为正常组、模型组,每组3只,采用60d单纯自由饮酒建立急性酒精性脂肪肝病证结合食蟹猴模型。每日观察食蟹猴的外在宏观表征,制定中医证候积分标准,对每只食蟹猴进行中医证候综合评分。采用全自动生化分析仪检测血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆固醇(TC)、甘油三酯(TG)的含量,应用ELISA检测血清胃泌素(GAS)、胃动素(MTL)含量,肝脏B超及HE染色观察肝组织病理学变化,计算肝指数变化。结果:与正常组比较,模型组第60天时血清ALT、AST、TC、TG、GAS、MTL含量及肝指数均显著升高(P<0.05,P<0.01),肝脏B超、HE染色显示模型组肝组织出现弥漫性脂肪样病变,中医证候积分显示该模型为脾胃湿热证模型。结论:60d梯度饮酒可以成功建立急性酒精性脂肪肝脾胃湿热证食蟹猴模型,该模型符合人类酒精性脂肪肝病理特点和中医脾胃湿热证证候特点,是较为理想的酒精性脂肪肝脾胃湿热证模型。

Preliminary study on establishment and evaluation of spleen-stomach damp-heat syndrome model of cynomolgus monkey with acute alcoholic fatty liver

Objective: To provide research tools for the study of alcoholic fatty liver in modern Chinese medicine, the model of acute alcoholice fatty liver of cynomolgus monkeys combined with disease and syndrome is established. Methods: Six healthy male cynomolgus monkeys were divided into normal group and model group, with 3 cynomolgus monkeys in each group. Establishment of acute alcoholic fatty liver disease syndrome combined with cynomolgus monkey model with 60-day pure free drinking. The external macroscopic characterization of cynomolgus monkeys was observed daily. TCM syndrome scores were established at the same time, and each comprehensive score of TCM syndromes was obtained for each cynomolgus monkey.The serum level of alanine transaminase(ALT), aspartate amino transferase(AST), total cholesterol(TC), triglyceride(TG) were detected by automated biochemical analyzer. Serum levels of gastrin(GAS) and motilin(MTL) were detected by ELISA. Liver B-ultrasond and HE staining were used to observe the pathological changes of liver tissue, and calculate changes in liver index. Results: Compared with the normal group, the serum levels of ALT, AST, TC, TG, GAS, MTL and liver index in the model group were significantly increased at 60 days(P<0.05, P<0.01). Liver B-ultrasound and HE staining showed diffuse fatty-like lesions in the liver tissue of the model group, and TCM syndrome scores showed that the model was a model of spleen-stomach dampheat syndrome. Conclusion: 60-day gradient drinking can successfully establish spleen-stomach damp-heat syndrome model of cynomolgus monkey with acute alcoholic fatty liver. This model is in line with the pathological characteristics of human alcoholic fatty liver and the syndrome characteristics of spleen and stomach damp-heat syndrome. It is an ideal damp-heat syndrome model of alcoholic fatty liver spleen and stomach.