苏丹红Ⅰ对人淋巴细胞基因表达谱的影响

目的利用基因芯片技术,研究苏丹红Ⅰ对外周血淋巴细胞基因表达谱的作用,着重探索其对人类肿瘤形成的影响。方法用10-5mol/L苏丹红Ⅰ处理人外周血淋巴细胞8h,提取实验组和对照组细胞mRNA,经逆转录和标记后与人类全基因组芯片(约35000个点)杂交。芯片扫描后提取并处理数据,筛选出与肿瘤发生相关的基因,进行生物信息学分析。结果306个表达上调的基因中有11个与肿瘤形成相关的基因;表达下调的181个基因中有16个与肿瘤形成相关的基因。结论苏丹红Ⅰ可引起淋巴细胞基因表达谱的变化,其中对肿瘤相关基因表达有影响,提示苏丹红Ⅰ有诱发肿瘤的倾向。

Gene Expression Profile of Sudan Ⅰ-Treated Peripheral Blood Lymphocytes

Objective Microarray was used to study the toxic effect of Sudan I on the gene expression profile of human lymphocytes, with emphasis on the gene expression and tumorigenesis.Methods Peripheral blood lymphocytes were treated with 10 -5 mol/L Sudan I for 8 h. The total RNA from cells of the subjects of the treated group and the control group were isolated, then they were reversely transcribed and labeled with fluorescence dyes, and finally hybridized with human whole genome microarray (about 35,000 spots). Microarray data was extracted after microarray scanning, and the genes correlating to tumor were screened out and analyzed by bioinformatics.Results Among 306 up-regulated genes, 11 genes were found to be related to tumor formation. 181 genes were down-regulated, of which 16 genes were related with tumor development.Conclusion Sudan I could affect gene expression of lymphocytes, and reduced gene expression is an indicator of tumor development.