人重组骨形成蛋白—2生物学活性及其载体的实验研究

人重组骨形成蛋白—２（ｒｈＢＭＰ－２）主要生物学作用是诱导新骨形成和促进骨缺损的修复。采用小鼠肌袋模型，观察了大肠杆菌表达的ｒｈＢＭＰ－２在不同剂量下的异位成骨活性。实验结果表明，ｒｈＢＭＰ－２具有较强的骨诱导活性，其生物学作用具有时间依赖性和剂量依赖性。另外还首次报道了将经综合化学处理的小羊骨松质作为ｒｈＢＭＰ－２的载体，使两者有机结合，研制成一种新型复合异种骨，并经小鼠异位诱导实验证实，经综合化学处理的骨松质是ｒｈＢＭＰ－２的良好载体，能够显著提高ｒｈＢＭＰ－２的骨诱导活性。

Bioactivity of rhBMP 2 and carrier

Human recombinant bone morphogenetic protein 2 (rhBMP 2) can induce new bone formation and be used for the treatment of bone defect. Bioactivity assay in mouse thigh muscle pouch showed that rhBMP 2 induced the proliferation of mesenchymal type cells and the fomation of new bone in the implantation area in a dose and time dependent manner. Comprehensively chemically treated lamb cancellous bone was combined with rhBMP 2 and implanted into the muscle pouch. The composited graft showed a definitive osteoinductive activity.The data indicate that the treated lamb bone can be used as the carrier and framework for rhBMP 2.