人椎间盘髓核细胞突起的形态学特征

目的 探讨成人腰椎间盘髓核细胞突起的形态学特征.方法 取8例成人腰椎间盘髓核组织标本(Thompson Ⅰ～Ⅱ),分别行冰冻切片和电镜切片,同时进行髓核细胞的分离和单层培养,利用光镜、激光扫描共焦显微镜和透射电镜,从组织、细胞和超微结构水平观察细胞突起的形态学特征.结果 所有的髓核细胞均具有明显的突起结构,相邻的细胞突起间可见缝隙连接.在体状态下均呈类圆形的髓核细胞进行离体培养时却呈现梭形和类圆形两种不同的形态,梭形细胞与类圆形细胞的比例约为2.3∶1.梭形细胞的突起顺着细胞体长轴发出,未见二级突起.类圆形细胞的突起从细胞体四周发出,突起呈树枝状,可见多级突起.结论 突起是椎间盘髓核细胞的形态学特征之一,对突起功能的深入研究将有助于加深对椎间盘退变病理机制的认识.

MORPHOLOGIC CHARACTERISTICS OF PROCESSES OF NUCLEUS PULPOSUS CELLS IN ADULT HUMAN INTERVERTEBRAL DISC

Objective To explore morphologic characterizatics of cellular processes from adult human nucleus pulposus cells. Methods The nucleus pulposus of adult human intervertebral disc were obtained from 8 patients(Thompson’s grade Ⅰ Ⅱ) and then the tissues specimens were carried out by frozen section and electron microscopic section as well as cell isolation and cultured, processes of nucleus pulposus cells were examined using light microscopy, laser scanning confocal microscopy and transmission electron microscopy. Results When examined at both the confocal and electron microscope level, all the cells possessed the processes and adjacent nucleus pulposus cells processes possessed a gap junction. The elongated and round cells were examined when NP cells became monolayer in vitro. The rate of elongated cells to round cells was 23 to 1. The elongated cells protruded along with the long axis of cell body without second processes. Dendritic processes of round cells protruded to all directions from the cell body with multiple level processes.Conclusion Processes are one of the morphologic characteristics of intervertebral disc cells. The research on proc