| Abstract: | Objective To study the effect and mechanism of mexican tea herb and pilular adina herb (abbreviated to MP) on concrescence of gastric
mucosa in experimental gastric ulcer rats by observing the changes of epidermal growth factor (EGF), nitrogen monoxidum (NO)
and expression of epidermal growth factor receptor (EGFR).
Methods The rat ulcer model was established by 100% glacial acetic injection into the subserosa. The ulcer index (UI) was measured
by sliding caliper. The levels of NO and EGF in tissue and serum were measured by the nitrate reductase method and enzyme-linked
immunosorbent assay, respectively. The expression of EGFR in the mucosa around the ulcer was detected by the immunohistochemical
assay and microimage analysis system.
Results (1) Compared with the model group, UI of MP groups (10, 15 and 20 mg·kg−1·d−1) and ranitidine group was lower (P<0.05 or P<0.01), the levels of NO and EGF in the tissue and serum were higher (P<0.05), the thickness of regenerated mucous membrane increased, and the width loss of lamina muscularis mucosa decreased (all
P<0.05). (2) The expression of EGFR is weakly positive in gastric mucosa cells in the normal group, mainly in the cytoplasm
and cytomembrane. In the model group, the expression of EGFR was mainly in epithelial cells in cervical part and basilar part
of gastric gland around the ulcer margin, and the number of cells with EGFR weakly positive expression was more than that
in the normal group. Compared with that in the normal and model groups, the number of cells with EGFR positive in MP groups
and ranitidine group increased (all P<0.05), with weakly positive expression.
Conclusion MP can protect gastric mucosa, cure gastric ulcer, restrain the secretion of gastric acid, and boost multiplication, differentiation,
migration and repair of the endothelial cell by promoting the secretion of NO and EGF, and increasing the expression of EGFR
of gastric mucosa epithelial cells. |
