多花黄精内生菌群落结构多样性及其与有效成分含量相关性研究

目的通过分析多花黄精Polygonatumcyrtonema内生菌菌群结构多样性及其与主要有效成分含量之间的相关性,探讨多花黄精内生菌对其药材品质的影响。方法收集不同产地多花黄精样品,采用高通量测序对内生菌群落结构进行分析,并测定多糖、总皂苷等有效成分含量,通过双因素关联分析探究菌群多样性及其与有效成分之间的关系。结果 5个产地15组多花黄精样品共得到1 183 635条有效序列,划分为59 474个操作分类单元(operational taxonomic units,OTU),其中细菌分19门,47纲,120目,201科,419属;属水平上,泛菌属为优势菌群,最大占比达到61.1%。真菌分7门,27纲,70目,132科,187属;属水平上,子囊菌门一未鉴定属、赤壳属为优势菌群,其中赤壳属的最大占比达到10.7%。在丰度前10的内生真菌和细菌中,共有10个菌属与有效物质含量呈显著相关,其中真菌有2个菌属呈负相关,1个菌属呈正相关;细菌2个菌属呈负相关,5个菌属呈正相关。结论通过分析不同产地多花黄精内生菌群落结构及其多样性,掌握了多花黄精内生菌资源状况,分析发现内生菌与有效成分之间存在关联,这为深入探讨多花黄精内生菌与其品质的相关性,进而采用生物施肥策略提升品质等提供了科学依据。

Study on community and diversity of endophyte and its correlation with active ingredient content in Polygonatum cyrtonema

Objective To explore the effect of endophyte on the quality of Polygonatum cyrtonema by analyzing the correlation between community and diversity of endophyte and the content of active ingredient. Methods P. cyrtonema from different locations were collected; High-throughput sequencing technology was used to analyze endophyte and the content of active ingredient were determined, including polysaccharides, total saponins and so on. The relationship between the diversity of endophyte and the active components was explored through two-factor correlation analysis. Results The results showed that 1 183 635 effective sequences were obtained from 15 samples of P. cyrtonema in five different places, which were divided into 59 474 operational taxonomic units (OTUs). The endophytic bacteria were divided into 19 phyla, 47 classes, 120 orders, 201 families, and 419 genera among them. At the genus level, Pantoea was the dominant genus, which the largest proportion amounted to 61.1%. The endophytic fungi were divided into 7 phyla, 27 classes, 70 orders, 132 families, and 187 genera. At the genus level, an unidentified genus from Ascomycota and Neocosmospora were the dominant genus, the largest proportion of Neocosmospora reached 10.7%. Among the abundance ratio of the top 10 endophytic fungi and bacteria, a total of 10 genera were significantly correlated with the content of active ingredients, which were two negative correlation and one positive correlation in fungi genus. Two and five genera of bacteria were negatively and positively correlated, respectively. Conclusion The results master the endophyte resources of P. cyrtonema by analyzing the community and diversity of endophyte from different habitat samples. It is showed that there is an interaction between endophyte and active ingredient. The present analysis provides a scientific basis for in-depth discussion of the interaction mechanism between endophyte and character of P. cyrtonema, and the use of biological fertilization strategies to improve quality in the future.