HPLC法分析闽产北沙参3种香豆素含量

目的了解闽产北沙参样品中3种香豆素成分的含量,为野生种质资源的保护与栽培种质资源的筛选提供基础数据。方法高效液相色谱法(HPLC法)。结果22批北沙参样品中,3种香豆素含量之和平均为198.62μg/g,其中补骨脂素含量(平均含量152.14μg/g)大大高于欧前胡素(平均含量21.94μg/g)与异欧前胡素(平均含量25.54μg/g),为二者含量的6倍以上;8月份后采集的、不同规格北沙参样品中,根直径为0.5~1 cm的中规格样品,3种香豆素含量均高于其他2种规格(直径<0.5 cm与直径>1 cm)的样品。结论闽产北沙参活性成分含量较高,在保护野生种质资源的基础上,筛选优质种质,推广闽产北沙参药材合理利用具有较大的应用前景。

Content Analysis of Three Coumarins in Glehniae Radix from Fujian Province by HPLC

Objective To know contents of three coumarins in Glehniae Radix from Fujian and provide basic data for protecting wild germplasm resources and screening culture germplasm resources.Methods High-performance liquid chromatography(HPLC)was applied.Results Among the 22 samples,the sum of the three coumarins was 198.62 μg/g on average,the content of psoralen(152.14 μg/g on average)was much higher than that of imperatin(21.94 μg/g on average)and isoimperatorin(25.54 μg/g on average),which was more than 6 times of that of both.Among the samples collected after August with different specifications,the intermediate specification with root diameter is 0.5-1.0 cm,whose contents of three coumarins were higher than those of the other two specifications(diameter<0.5 cm and diameter>1.0cm).Conclusion The contents of active ingredient in Glehniae Radix from Fujian were higher.On the basis of protecting wild germplasm resources,selecting high-quality germplasm and promoting their rational utilization are of great application prospects.