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| 逆转录病毒载体介导HPV16 E6E7基因转化人胚食管纤维细胞的研究 | |
| 引用本文： | 张拥军,郭秀婵,张永利,赵健,沈忠英,曾毅. 逆转录病毒载体介导HPV16 E6E7基因转化人胚食管纤维细胞的研究[J]. 中华实验和临床病毒学杂志, 2004, 18(3): 223-226 |
| 作者姓名： | 张拥军 郭秀婵 张永利 赵健 沈忠英 曾毅 |
| 作者单位： | 1. 100052,北京,中国疾病预防控制中心病毒病预防控制所肿瘤与艾滋病室 2. 四川大学华西医学院附属第二医院 3. 汕头大学医学院病理教研室 |
| 基金项目： | 国家自然科学基金重点资助项目 ( 3 983 0 3 80 ) |
| 摘 要： | 目的 研究HPV16型病毒感染与食管癌发生的关系。方法 包装含有HPVl6E6E7基因重组逆转录病毒，以重组病毒感染人胚食管纤维细胞，在TPA协同下诱导SCID小鼠成瘤。结果 重组病毒感染人胚食管纤维细胞可以诱导SCID小鼠形成肉瘤，可以检测到E6E7基因的存在及表达，流式细胞仪检测从瘤组织培养出来的纤维细胞，确定为异倍体；但未能诱导人胚食管组织成瘤。结论 建立的重组逆转录病毒系统可以成功介导HPV16E6E7基因的转移，可以应用于HPV致瘤性研究。 |
| 关 键 词： | E7基因 食管 人胚 HPV16 纤维细胞 介导 SCID小鼠 重组病毒 毒感染 诱导 |
| 修稿时间： | 2004-02-05 |
Studies on transformation of human esophageal fibroblasts mediated by a retroviral vector containing the E6E7 ORFs of human papillomavirus type 16 |
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| ZHANG Yong-jun ,GUO Xiu-chan,ZHANG Yong-li,ZHAO Jian,SHEN Zhong-ying,ZENG Yi. Studies on transformation of human esophageal fibroblasts mediated by a retroviral vector containing the E6E7 ORFs of human papillomavirus type 16[J]. Chinese journal of experimental and clinical virology, 2004, 18(3): 223-226 | |
| Authors: | ZHANG Yong-jun GUO Xiu-chan ZHANG Yong-li ZHAO Jian SHEN Zhong-ying ZENG Yi |
| Affiliation: | Department of Oncogenic Virus and HIV, National Institute for Viral Disease and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China. |
| Abstract: | Objective To study the etiological role of human papillomavirus type 16 (HPV16) infection in the development of esophageal cancers Methods A recombinant retrovirus containing the E6E7 ORFs of HPV16 was packaged and human fetal esophageal fibroblasts were infected The tumorigenecity of the fibroblasts was tested in SCID mice in synergy with 12-O-tetradecanoylphorbol-13-acetate (TPA) Results Human esophageal fibroblasts infected with the recombinant retrovirus induced sarcomas in SCID mice, the existence and expression of E6E7 ORFs was confirmed in the sarcomas Fibroblasts cultured from the sarcoma were demonstrated heteroploid by cytoflowmetry However, tumors were not observed in human fetal esophagus infected with such virus Conclusion These results revealed that the established recombinant retroviral system can successfully mediate the transference of HPV16 E6E7 genes, and such system is applicable to researches on tumorigenesis of HPV |
| Keywords: | Papilomavirus human Retrovirdae Fibroblasts Oncogenes |
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