| 原生质体紫外诱变技术筛选红霉素高产菌株的研究 |
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| 引用本文： | 王筱虹,倪文琴,刘寒.原生质体紫外诱变技术筛选红霉素高产菌株的研究[J].中国药科大学学报,2000,31(4):63-65. |
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| 作者姓名： | 王筱虹 倪文琴 刘寒 |
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| 作者单位： | [1]上海万兴生物制品有限公司 [2]中国药科大学生物制药学院 |
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| 摘 要： | 将红霉素产生菌－红色链霉菌的菌丝体经溶菌酶２壁后获得的原生质体，用紫外线照射一定时间后，在再生培养基上得到再生菌株，通过活性测定，挑选抑菌圈大而清晰的再生菌株进行摇瓶筛选，最后得到比对照菌株效价提高２２５．８％的高产菌株ＨＰ－３。
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| 关 键 词： | 红霉素 原生质体 紫外诱变 高产菌株 |
| 修稿时间： | 1999-08-30 |
Screening High-yield Strains of Streptomyces erythreus by UV Mutagenesis |
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| WANG Xiao-Hong,NI Wen-Qing,LIU Han.Screening High-yield Strains of Streptomyces erythreus by UV Mutagenesis[J].Journal of China Pharmaceutical University,2000,31(4):63-65. |
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| Authors: | WANG Xiao-Hong NI Wen-Qing LIU Han |
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| Institution: | WANG Xiao-Hong
,NI Wen-Qing
,LIU Han
(Department of Microbial Pharmaceutics, China Pharmaceutical University, Nanjing 210009) |
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| Abstract: | Using lysozyme action on the mycelia, the Streptomyces erythreus protoplasts were obtained, then treated by UV light for certain time, the mutated strains were selected by potency determination after shake flasks fermentation, and was obtained a high yield strains HP 3, and the potency was 225.8% that of original strains. |
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| Keywords: | Erthymycin Protoplasts UV Mutagenesis |
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