铁蛋白与SPIO在磁共振细胞活体示踪中的增效作用

目的研究C6细胞内高表达的铁蛋白与标记的超顺磁性纳米铁颗粒（SPIO）的相互作用,利用磁共振进行此类标记细胞的活体示踪。方法构建鼠铁蛋白重链基因质粒并转染鼠胶质瘤C6细胞。转染铁蛋白基因的细胞和对照细胞标记SPIO后,分别皮下接种于一组裸鼠后腿部左、右侧。在肿瘤细胞接种后不同时间点,进行T2WI扫描。接种2周后处死裸鼠,测定两组接种细胞生成的肿瘤组织中的铁浓度。结果进行铁蛋白基因转染并标记SPIO的C6细胞来源肿瘤,铁浓度明显高于没有铁蛋白基因转染但标记SPIO的C6细胞来源肿瘤（P=0.034,n=5）。在接种后第2天（P=0.012,n=5）、第6天（P=0.003,n=5）和第13天（P=0.021,n=5）,进行铁蛋白基因转染并标记SPIO的C6细胞来源肿瘤与对照组相比,T2信号强度明显改善。结论铁蛋白与SPIO相互作用,并可以延长磁共振活体细胞示踪时间。

Ferritin coordinates with SPIO in tracking C6 rat glioma cells by MRI

Objective：To investigate the effect of ferritin protein overexpression on superparamagnetic iron oxide （SPIO） particle labeling of C6 rat glioma cells,and to track the labeled cells in vivo using magnetic resonance imaging （MRI）.Materials and Methods：Plasmid of H-chain of murine ferritin gene was constructed and transfected into C6 cells.The parental and transfected C6 cells labeled with SPIO were bilaterally inoculated subcutaneously into nude mice.The mice were subjected to multiple T2-weighted MR scans after C6 cell inoculation.The mice were sacrifi ced in two weeks and the concentration of iron in the tumor tissue was measured.Results：The concentration of iron in xenografts derived from ferritin plasmid transfected and SPIO labeled C6 cells was significant higher than that in xenografts from parental C6 cells labeled with SPIO （P=0.034,n=5）.Ferritin transfected C6 cells with SPIO labeling showed an improved T2 contrast in vivo after inoculating of 2 days （P=0.012,n=5）,6 days （P=0.003,n=5）,and 13 days （P=0.021,n=5）.Conclusion：Coordinating ferritin with SPIO can lead to a longer MRI cellular tracking period.