Integrated analysis of transcriptomics and metabonomics profiles in aflatoxin B1-induced hepatotoxicity in rat |
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| Affiliation: | 1. Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China;2. Shanghai Institute for Food and Drug Control, Department of Traditional Chinese Medicine, Shanghai 201203, China;1. Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China;2. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, PR China;3. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China;4. China National Research Institute of Food and Fermentation Industries, Beijing 100027, PR China;1. Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China;2. Department of Animal Science, Cornell University, Ithaca, NY 14853, USA;1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Food Safety and Quality Control of Jiangsu Province, Jiangnan University, Wuxi 214122, PR China;2. Centre for Sustainable Nanotechnology, School of Chemical & Life Sciences, Nanyang Polytechnic, Singapore 569830, Singapore;1. Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China;2. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China;3. Department of Animal Science, Cornell University, Ithaca, NY 14853, USA;1. Institute of Neuroscience and Psychology, Glasgow University, Glasgow G12 8QQ, UK;2. Histology Departments, Faculty of Medicine, Tanta University, Tanta 31527, Egypt;3. Histology Department, Faculty of Medicine, Sohag University, Sohag 82524, Egypt;4. Pharmacology Department, Faculty of Medicine, Tanta University, Tanta 31527, Egypt |
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| Abstract: | The aim of this work was to identify mechanisms and potential biomarkers for predicting the development and progression of aflatoxin B1 (AFB1)-induced acute hepatotoxicity. In this study, microarray analysis and metabolites profiles were used to identify shifts in gene expression and metabolite levels associated with the affected physiological processes of rats treated with AFB1. Histopathological examinations and serum biochemical analysis were simultaneously performed; the results indicated that hepatotoxicity occurred in higher dosage groups. However, gene expression analysis and metabolite profiles are more sensitive than general toxicity studies for detecting AFB1-induced acute hepatotoxicity as the patterns of low-dose AFB1-treated rats in these two technique platforms were more similar to the rats in higher dosage groups than to the control rats. Integrated analysis of the results from general toxicity studies, transcriptomics and metabonomics profiles suggested that p53 signaling pathway induced by oxidative damage was the crucial step in AFB1-induced acute hepatotoxicity, whereas gluconeogenesis and lipid metabolism disorder were found to be the major metabolic effects after acute AFB1 exposure. The genes and metabolites significantly affected in common in rat liver or serum of three doses AFB1 treatments served as potential biomarkers for detecting AFB1-induced acute hepatotoxicity. |
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