先天性心脏病心脏组织中TORCH病原基因的检测

目的 探讨先天性心脏病（CHD）心肌组织中TORCH各病原体（弓形体／TOX；风疹病毒／RV；单纯疱疹病毒／HSV2；巨细胞病毒／CMV）的感染状况。方法 通过收集42例先天性心脏病及38例对照组的心脏组织石蜡标本，用聚合酶链反应（PCR）或逆转录－聚合酶链反应（RT－PCR）技术进行病毒基因的检测。结果 在42例先天性心脏病心肌组织中检测到CMV、HSV、TOX的阳性率分别为26.2%、4.7%和16.7％，而例对照组中CMV、HSV2、TOX的阳性率分别为21.1%、2.6%和2.6%，丙组比较TOX在两组中的检测差异有显著性（P＝0.0378），而CMV、HSV2在两组中无统计学意义（P＞0.05）。在30例先天性心脏病中RV的阳性率为23.3%，而20例对照均有性，两组间差异有显著性（P＝0.0328）。结论 从先天性心脏病的心脏组织中发现有TORCH病原体TOX、RV、CMV和HSV2基因存在，为从分子水平进一步进行TORCH病原感染与先天性心脏病关系的研究打下基础。

Detection of TORCH genom in the cardiac tissue of congenital heart disease

OBJECTIVE: To explore relationships of TORCH (cytomegalovirus/CMV, toxoplasma/TOX, rubella virus/RV, herpes simplex virus-2 HSV2) infection with congenital heart disease (CHD). METHODS: We conducted comparison to detection TORCH pathogenic gene in 42 cases of CHD and 38 controls by polymerase chain reaction (PCR). RESULTS: 1. The positive rate of CMV, HSV2 and TOX in 66 cases of CHD were 26.2%, 4.7% and 16.7%, respectively, while those of 38 cases in control groups were 21.1%, 2.6% and 2.6%, respectively. The infection condition of TOX was significantly different (P = 0.0378), while those of CMV and HSV were not statistically different (P > 0.05) between CHD and control groups. 2. 7 of 30(23.3%) were positive for RV-RNA, compared with control group which were all negative, it was significantly higher (P = 0.0328). CONCLUSION: This is the first report of the presence of TORCH genes in CHD cardiac tissue by PCR. It might lay the foundation for studying the relationship of TORCH infection with CHD in molecular level.