肾癌患者血清中可溶性B7-H4表达及临床意义

目的:检测肾癌患者血清中可溶性B7-H4(sB7-H4)的表达水平,与相关临床指标比较,探讨临床意义。方法:应用ELISA法检测肾癌患者40例与非肾癌患者10例血清中sB7-H4浓度,根据肿瘤分期、分级及T细胞亚群结果进行分类分组。结果:(1)两组血清sB7-H4水平比较:肾癌组为(23.08±19.42)μg/L,对照组为(11.41±5.48)μg/L,两者比较差异有统计学意义(P<0.01)。(2)血清sB7-H4水平临床分期比较:肾癌I、II、III/IV期分别为13.24±9.08μg/L、26.99±10.89μg/L、53.63±27.22μg/L,三者比较差异有统计学意义(P<0.01)。(3)血清sB7-H4水平病理分级比较:I、II、III级分别为10.08±5.07μg/L、23.01±10.20μg/L、52.47±20.53μg/L,三者比较差异有统计学意义(P<0.01)。且肾癌患者血清sB7-H4水平与T细胞亚群分析呈线性负相关。结论:肾癌患者血清sB7-H4水平显著增高,且与病理分型、临床分期呈正相关,与血T细胞亚群分析等情况呈线性负相关。提示sB7-H4与肾癌发生、发展有关,检测sB7-H4对肾癌的诊断及病情评估有一定的意义。

Detection and Significance of Soluble B7-H4 in Renal Cell Carcinoma

Objective：To detect soluble B7-H4（sB7-H4）in serum of renal cell carcinoma patients and discuss its clinical significance.Method：Sandwich ELISA was applied to test sB7-H4 in serum of 40 renal cell carcinoma patients and 10 healthy controls.And in accordance with tumor stages,grades and T cell subsets results of the analysis groups are categorized compared with the groups of serum sB7-H4 differences in order to explore its clinical significance.Result：Serum sB7-H4 in renal cell carcinoma patients was 21.57±12.13μg/L whereas healthy controls＇ was 11.41±5.48μg/L.The difference between them showed significance（P0.01）.The sB7-H4 serum levels of renal cell carcinoma I,II,III / IV patients were 13.24±9.08μg/L,26.99±10.89μg/L and 53.63±27.22μg/L respectively,and the difference between the three groups was significant（P0.01）.The sB7-H4 serum levels of renal cell carcinoma of patholosical grade I,II,III class were 10.08±5.07μg/L,23.01±10.20μg/L and 52.47 ± 20.53μg/L respectively,and the difference between the three group was significant（P0.01）.And renal cell carcinoma patients＇ sB7-H4 levels and T cell subsets were in linear negative correlation（P0.01）.Conclusion：Serum sB7-H4 of renal cell carcinoma patients increases significantly and positively correlates with pathological classification and clinical stages.And renal cell carcinoma patients＇ sB7-H4 levels and T cell subsets were in linear negative correlation.The result implicates that sB7-H4 correlates with the occurrence and development of renal cell carcinoma.Detecting on sB7-H4 is helpful for renal cell carcinoma diagnosis and condition assessment.