痛风宁胶囊对小鼠高尿酸血症和肾脏功能的改善作用

目的：探讨痛风宁胶囊（TFN）对高尿酸血症模型小鼠血清中尿酸（UC）、肌酐（Cr）和尿素氮（BUN）水平以及小鼠肝脏匀浆液中黄嘌呤氧化酶（XOD）和腺苷脱氨酶（ADA）活性的影响，观察TFN对高尿酸小鼠肾脏组织病理学变化的改善作用，阐明其作用机制。方法：采用酵母膏合并氧嗪酸钾盐诱导建立小鼠高尿酸血症模型。将70只小鼠分为空白对照组，模型组，低（200 mg·kg^-1）、中（400 mg·kg^-1）和高剂量（800 mg·kg^-1） TFN组，别嘌醇（50 mg·kg^-1）和痛风舒（TFS，600 mg·kg^-1）阳性药对照组，每组10只，灌胃给药。实验结束后检测小鼠血清中UC、Cr和BUN水平以及小鼠肝脏匀浆液中XOD和ADA活性，HE染色观察小鼠肾脏组织病理表现。结果：与空白对照组比较，模型组小鼠血清中UC、Cr和BUN水平明显升高（P < 0.01），肝组织中XOD和ADA活性亦升高（P < 0.01）；与模型组比较，阳性药对照组，低、中和高剂量TFN组小鼠血清中UC、Cr和BUN水平明显降低（P < 0.01），肝组织中XOD和ADA活性降低（P < 0.05或P < 0.01），其中高剂量TFN组效果最为明显。光镜下观察，与模型组比较，阳性药对照组和高剂量TFN组小鼠肾脏肾小球萎缩、肾间质纤维化和肾小管扩张等病理变化得到一定程度上改善。结论：TFN对小鼠高尿酸血症具有一定的改善作用，其机制与抑制UC生成和促进UC排泄有关。

Improvement effects of Tongfengning Capsule on hyperuricemia and renal function in mice

Objective:To discuss the influence of Tongfengning Capsule (TFN) in the levels of uricacid (UC),creatinine (Cr) and urea nitrogen (BUN) in mouse serum and the activities of the xanthine oxidase (XOD),adenosine deaminase (ADA) activity in the liver homogenate of the mice with hyperuricemia,and to observe the improvement effect of TFN on the pathological changes of liver tissue and to clarify its mechanisms.Methods:The models of mouse hyperuricemia were induced by yeast extract with potassium oxonate.Seventy mice were divided into blank control group,model group,low (200 mg · kg 1),medium (400 mg · kg-1) and high (800 mg · kg-1) doses of TFN groups,allopurinol positive drug control group (50 mg · kg-1),Tongfengshu (TFS,600 mg · kg-1) positive drug control group (n=10).The levels of UC,Cr,BUN in serum and the activities of XOD,ADA in homoggenate were detected and the histopathological changes of the kidney tissue of the mice were measured with HE staining.Results:Compared with blank control group,the levels of serum UC,Cr and BUN ofthe mice in model group were significantlyincreased (P＜0.01),and the activities of XOD and ADA in liver tissue were also increased (P＜0.01).Compared with model group,the levels of serum UC,Cr and BUN of the mice in positive drug control groups and different doses of TFN groups were decreased (P＜0.01),and the activities of XOD and ADA in liver tissue were also decreased (P＜0.05),especially in high dose of TFN group.Compared with model group,the pathologic changes such as renal glomerulus atrophy,renal interstitial fibrosis and expansion of renal tubule of the mice in positive drug control groups and high dose of TFN group were improved to a certain extent.Conclusion:TFN has improvement effcet on the hyperuricemia in the mice and its mechanism is related to the inhibition of uricogenesis and the promotion of UC excretion.