肾炎康复片对糖尿病肾病大鼠内质网应激的影响

目的:探讨肾炎康复片对糖尿病肾病大鼠内质网应激的影响。方法:30只雄性SD大鼠随机分为3组:正常对照组(n=10);糖尿病肾病组(n=10);肾炎康复片组(n=10)。16周后检测大鼠24 h尿蛋白及血肝肾功水平。光镜观察大鼠肾组织病理改变。免疫组化方法检测大鼠肾脏葡萄糖调节蛋白78(glucose regulated protein 78,GRP78)、p-JNK的表达。TUNEL检测肾组织凋亡细胞。RT-PCR检测肾组织GRP78 mRNA、CHOP mRNA的表达。结果:18周时,糖尿病肾病组、肾炎康复片组24 h尿蛋白水平、血清尿素及肌酐水平均显著高于正常对照组(P<0.05),血白蛋白水平低于正常对照组。肾炎康复片组血清尿素及肌酐水平较糖尿病肾病组明显降低。糖尿病肾病组、肾炎康复片组GRP78、JNK、生长阻滞和DNA诱导基因153(growth arrest and DNA damage-inducible gene 153,CHOP)表达显著高于正常对照组(P<0.05),肾炎康复片组较糖尿病肾病组表达明显降低(P<0.05)。结论:内质网应激参与了糖尿病肾病的发生,肾炎康复片可以通过抑制肾脏的内质网应激反应而起肾脏保护作用。

The effect of Shenyankangfu tablets on endoplasmic reticulum stress in diabetic nephropathy rats

Objective:To investigate the effect of Shenyankangfu tablets on endoplasmic reticulum stress in diabetic nephropathy rats.Methods:30 male SD rats were randomly divided into three groups:normal control group (n=10),diabetic nephropathy group (n=10) and Shenyankangfu tablets group (n=10).24 h after 16 weeks,the levels of urine protein and serum hepatic and renal function were detected.The pathological changes of renal tissue in rats were observed with light microscope.The expression of glucose regulated protein 78(GRP78) and p-JNK in rat kidney was detected by immunohistochemistry method.Detection of apoptosis cells in renal tissues with TUNEL.The expression of mRNA GRP78 and mRNA CHOP in renal tissues was detected by RT-PCR.Results:At 18 weeks,diabetic nephropathy group,Shenyankangfu tablets group 24 h urine protein level,serum urea and creatinine levels were significantly higher than the normal control group (P<0.05),serum albumin level was lower than the normal control group.The serum urea and creatinine levels in Shenyankangfu tablets group were significantly decreased than the diabetic nephropathy group.The expression of GRP78,JNK,growth arrest and DNA damage-inducible gene 153(CHOP) in Shenyankangfu tablets group and diabetic nephropathy group was significantly higher than the normal control group (P<0.05),and those in Shenyankangfu tablets group was significanly decreased than the diabetic nephropathy group (P<0.05).Conclusion:The endoplasmic reticulum stress is involved in the occurrence of diabetic nephropathy,Shenyankangfu tablets can play a role in renal protection by inhibition of endoplasmic reticulum stress in the kidney