|
|||||
|
|
| 软肝冲剂对肝纤维化大鼠组织金属蛋白酶抑制剂的影响 | |
| 引用本文： | 莫其农,黄涛,方晓艺,周小梅,何进才,贺劲松,周小舟,童光东,熊益群,周大桥.软肝冲剂对肝纤维化大鼠组织金属蛋白酶抑制剂的影响[J].中西医结合肝病杂志,2008,18(3):160-163. |
| 作者姓名： | 莫其农 黄涛 方晓艺 周小梅 何进才 贺劲松 周小舟 童光东 熊益群 周大桥 |
| 作者单位： | 1. 广州中医药大学附属深圳市中医院检验科,广东,深圳,518033 2. 广州中医药大学附属深圳市中医院体检中心,广东,深圳,518033 3. 广州中医药大学附属深圳市中医院肝病研究所,广东,深圳,518033 |
| 基金项目： | 广东省深圳市科技局科研项目 |
| 摘 要： | 目的：探讨软肝冲剂治疗肝纤维化和肝硬化的机制是否与抑制TIMP-1的表达有关。方法：用CCl4诱导大鼠的肝纤维化模型（病理分期为0-Ⅴ期），将大鼠随机分成两组，分别用软肝冲剂和生理盐水治疗2个月后。对肝组织TIMP-1mRNA的表达进行分析，并检测羟脯氨酸（Hyp）的量，同时检测血清中TIMP-1和TGF-β1的浓度。结果：软肝冲剂能够降低大鼠肝组织中TIMP-1mRNA的表达和胶原蛋白的含量，同时血清中TIMP-1和TGF-β1较CCl4诱导的模型组和生理盐水治疗组显著降低（P〈0．05）。结论：软肝冲剂治疗肝纤维化可能通过阻止TIMP-1的表达起作用。 |
| 关 键 词： | 软肝冲剂/药理作用 组织金属蛋白酶抑制剂 肝纤维化 动物模型 |
Resolution of CCl4-induced rat liver fibrosis via TIMP-1 expression inhibition by Ruangan granule |
|
| Institution: | MO Qi-nong, HUANG Tao, FANG Xiao-yi, et al. (Laboratory Medicine, Shenzhen Traditional Chinese Medicine Hospital ( Shenzhen Guangdong, 518033) China) |
| Abstract: | Objective： To study the relation between the effect of Ruangan granule to liver fibrosis and tissue inhibitor of metalloproteinases-1 （TIMP-1） mRNA expression. Methods： CCl4-induced rats were randomly divided into two groups which were treated by Ruangan granule and Normal Saline （NS） respectively. The expression of TIMP-1 mRNA and hydroxyproline in liver tissue was analyzed. And serum levels of TIMP-1 mRNA and TGF-β1 were detected. Results： The expression of mRNA and collagen in liver tissue of CCl4-induced rats treated by Ruanganchengji were significantly decreased, the serum levels of TIMP-1 and TGF-β1 were significantly decreased compared with those of model and NS-treated groups. Conclusion： The mechanism of Ruangan granule treatment for liver fibrosis is possible to be blockade of TIMP-1 expression in liver. |
| Keywords: | Ruangan granule tissue inhibitor of metalloproteinases-1 （TIMP-1） liver fibrosis animal model |
| 本文献已被 维普 万方数据 等数据库收录！ | |