| HPLC法测定大黄微波提取物中大黄酸和番泻苷A的含量 |
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| 引用本文： | 钟晨.HPLC法测定大黄微波提取物中大黄酸和番泻苷A的含量[J].广东药学院学报,2009,25(5):483-485. |
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| 作者姓名： | 钟晨 |
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| 作者单位： | 南方医科大学,中医药学院,广东,广州,510515;广东药学院,药科学院,广东,广州,510006 |
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| 摘 要： | 目的建立大黄微波提取物中大黄酸、番泻苷A两种泻下成分的含量测定方法。方法采用HPLC法,色谱柱：Phenomenex Gemini C18柱（250 mm×4.6 mm,5μm）,流动相：甲醇-0.1%磷酸（体积比80∶20）,流速：1.0 mL/min,检测波长：254 nm,柱温：25℃。结果测定3批提取物,大黄酸和番泻苷A平均质量分数分别为7.24 mg/g和10.28 mg/g。结论本法简单快捷,结果可靠,可作为大黄提取物中大黄酸和番泻苷A的含量测定方法。
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| 关 键 词： | HPLC法 大黄提取物 大黄酸 番泻苷A |
Determination of rhein and sennoside A in extracts of rhubarb prepared with microwave extraction by HPLC |
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| ZHONG Chen.Determination of rhein and sennoside A in extracts of rhubarb prepared with microwave extraction by HPLC[J].Academic Journal of Guangdong College of Pharmacy,2009,25(5):483-485. |
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| Authors: | ZHONG Chen |
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| Institution: | ZHONG Chen1,2(1.Graduate of Southern Medical College,Guangzhou,Guangdong 510515,China,2.Guangdong Pharmaceutical College,Guangdong 510006,China) |
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| Abstract: | Objectlve To determine the contents of rhein and sennoside A in extracts of rhubarb prepared with microwave extraction . Methods Samples were analyzed on a Phenomenex Gemini C18 （250 mm×4.6 mm,5 μm） column using methyl alcohol-0.1% H3PO4 （80： 20） as mobile phase. The flow rate was 1.0 mL/min and UV detection wavelength was 254 nm. The column temperature was 25 ℃. Results Contents of rhein and sennoside A in the extracts of rhubarb were 7. 24 mg/g and 10. 28 mg/g, repectively. Conclusion The method is simple and reproducible, which could be used for the analysis of rhein and sennoside A in rhubarb extracts. |
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| Keywords: | HPLC rhubarb extracts rhein sennoside A |
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