应用微透析技术和质谱法测定川芎嗪对大鼠脑内乙酰胆碱释放量的影响

本文结合脑内微透析技术与现代分析方法，考察川芎嗪对大鼠脑内乙酰胆碱释放的影响。采用脑内微透析技术进行取样，建立高效液相-串联四极杆质谱方法测定脑透析液中的乙酰胆碱含量。结果显示，川芎嗪皮下给药能够剂量相关地增加大鼠脑内不同脑区中乙酰胆碱的释放。该法能够准确反映药物对大鼠脑内乙酰胆碱释放量的影响，与传统方法相比，具有明显的优势。

Effects of ligustrazine on extracellular acetylcholine levels in rat brain dialysate

Using brain microdialysis and LC-MS/MS to detect acetylcholine in rat brain to investigate the effects of ligustrazine. A liquid chromatography-tandem mass spectrometry method has been developed and validated for the determination of acetylcholine in rat brain dialysate sampling by microdialysis.The results indicated that ligustrazine administration by subcutaneous injection significantly increased Ach release in rat medial prefrontal cortex and nucleus accumbens in a dose-related manner.The drug's effect on Ach release in rat brain could be directly detected by microdialysis combined with HPLC-MS/MS and this method is selective and sensitive.